砂箱中柴油降解菌株的分离鉴定  

Isolation and Identification of Diesel Degradation Strain in Sandbox

作　　者：杨金凤[1] 曹冠楠[2] 杨正礼[1] 

机构地区：[1]中国农业科学院农业环境与可持续发展研究所,北京100081 [2]中国地质大学(北京)水资源与环境工程北京市重点实验室,北京100083

出　　处：《桂林理工大学学报》2011年第3期450-453,共4页Journal of Guilin University of Technology

基　　金：科技部国际科技合作项目(2006DFA21180)

摘　　要：以0#柴油为唯一碳源,从模拟生物修复柴油污染土壤的砂箱中筛选出4株柴油降解菌株B-1、B-2、B-3和B-4,根据形态学观察及16S rDNA序列比对分析,4株菌株分别属于微杆菌属(Microbacterium sp.)、短波单胞菌属(Brevundimonas sp.)、Tetrathiobacter kashmirensis、假单胞菌属(Pseudomonas sp.),且菌株B-3是2005年在克什米尔某处果园土壤中新分离出来的柴油降解菌株。此外,通过比较各菌株的生长曲线,菌株B-3和菌株B-4较其他两株菌优先进入对数生长期,对加快柴油的生物降解意义重大。Taking 0# diesel oil as the sole carbon source,four diesel-degrading strain（B-1,B-2,B-3 and B-4）are selected from the sand box of diesel contaminated soil.The results of morphological observation and identification of molecular biology show that these strains belong to Microbacterium sp.,Brevundimonas sp.,Pseudomonas sp.,Pseudomonas sp.,respectively.And strain B-3 is a new diesel-degrading strain first found in Kashmir orchard soil in 2005.In addition,strain B-3 and B-4 have preferentially accessed to the logarithmic growth phase than the other two strains through comparing the growth curve of each strain.It is significant for accelerating the biodegradation of diesel oil.

关 键 词：柴油 优势降解菌 16S rDNA测序 聚合酶链反应 

分 类 号：Q93-331[生物学—微生物学]