缬草挥发油与水提物镇静催眠作用比较研究  

Comparative Study on Sedative and Hypnotic Effects of Volatile Oil and Water Extract from Valerian

作　　者：丁菲[1] 方颖[1] 文莉[2] 刘焱文[1] 

机构地区：[1]湖北中医药大学中药资源与中药复方教育部重点实验室,武汉430065 [2]湖北中医药大学药理教研室

出　　处：《中国药师》2011年第10期1411-1413,共3页China Pharmacist

基　　金：国家自然科学基金(编号:30500053);湖北省教育厅科学技术研究计划(编号:Q20101806)

摘　　要：目的:了解缬草镇静催眠的主要药效物质,为深入研究其有效成分提供物质基础。方法:通过缬草挥发油急性毒性实验,了解挥发油毒性的大小,确定药效实验剂量。采用镇静催眠的药效学实验,比较缬草挥发油与水提物协同戊巴比妥钠对小鼠的催眠作用。结果:小鼠灌胃缬草挥发油的LD_(50)为278.99 g·kg^(-1)(以生药计),从而确定药效试验的给药剂量为高剂量组(85.71 g·kg^(-1))、中剂量组(57.14 g·kg^(-1))和低剂量组(28.57 g·kg^(-1));缬草镇静催眠药效学实验表明,缬草挥发油可促进小鼠入睡效率,延长小鼠睡眠时间;水提物仅可延长小鼠的睡眠时间,与水提物相比,缬草挥发油能有效延长小鼠的睡眠时间。结论:缬草挥发油的镇静催眠效果明显优于其水溶性物质。Objective： To understand the primary materials from valerian with sedative and hypnotic effects to provide basis for further research on the effective component. Method： Through the acute toxicity test, the toxicity of valerian volatile oil could be detected to decide the dosage in the efficacy trials. Through the sedative and hypnotic experiment, the cooperative effects of valerian volatile oil and water extract with pentobarbital sodium in mice were studied and compared. Result： The LD50 of valerian volatile oil in mice was 278.99 g·kg^-1 （calculated by crude drug） ,and the determined dosage in the efficacy trials was high dosage group of 85.71 g·kg^-1, middle dose group of 57. 14 g·kg^-1 and low dose group of 28. 57 g·kg^-1. Valerian volatile oil could obviously improve the sleep efficiency and prolonged the sleep time of mice. The water extract prolonged the sleep time only and valerian volatile oil showed more promising effect. Conclusion： The sedative and hypnotic effects of valerian volatile oil are obviously better than those of the water extract.

关 键 词：缬草挥发油 水提物 镇静催眠作用 

分 类 号：R285.5[医药卫生—中药学]