牙鲆GH基因第1外显子区微卫星标记与幼鱼生长性状的相关分析  

Correlation Analysis of Microsatellite DNA Marker in the GH Exon 1 Region with Growth Traits of Juvenile Olive Flounder Paralichthys olivaceus

作　　者：倪静[1] 尤锋[1] 刘思思 吴志昊[1] 徐冬冬[1] 文爱韵[1] 徐永立[1] 张培军[1] 

机构地区：[1]中国科学院海洋研究所实验海洋生物学重点实验室,青岛266071 [2]青岛易邦生物工程有限公司,青岛266032

出　　处：《动物学杂志》2011年第5期108-113,共6页Chinese Journal of Zoology

基　　金：国家973项目(No.2010CB126304);公益性行业(农业)科研专项经费项目(No.200903046);鲆鲽类产业技术体系:苗种繁育项目科学家课题(No.nycytx-50)

摘　　要：以牙鲆(Paralichthys olivaceus)一个养殖群体中的100个个体为研究材料,分析了其GH基因第1外显子区域中微卫星座位的多态性,同时对各基因型与其体重、体长等生长性状进行了关联性分析。结果表明,该外显子微卫星座位具有一定的多态性,共检测到3个等位基因(A、B、C)、5种基因型,这3个等位基因的频率分别为0.339、0.609和0.517;该座位的观测杂合度及预期杂合度分别是0.540 3和0.511 2,多态信息含量及有效等位基因数分别是0.423 8和2.045 8。One-way ANOVA统计结果显示,基因型为AC的个体体重、头长和体高明显大于其他基因型个体(P<0.05),C是一个对体重、头长和体高有利的等位基因。In the GH exon 1 region,a microsatellite locus was detected in 100 juvenile samples from a cultured stock of olive flounder,Paralichthys olivaceus.The polymorphism of this locus and the genetic effect of genotypes on economic traits in this stock were analyzed.The results indicated that this microsatellite locus was polymorphic.Five genotypes and three allele were tested.The frequency values of A,B and C alleles were 0.339,0.609 and 0.517,respectively.The observed heterozygosity and expected heterozygosity of this locus were 0.540 3 and 0.511 2.And the polymorphism information content and the effective allele number were 0.423 8 and 2.045 8,respectively.One-way ANOVA result showed that fish with genotype AC were heavier than those with other genotypes and C was an effective allele in flounder weight.

关 键 词：牙鲆幼鱼 GH基因第1外显子 微卫星 遗传多态性 生长性状相关 

分 类 号：S917.4[农业科学—水产科学]