金银花与山银花生物指纹图谱比较研究(英文)  

Comparison of microcalorimetric fingerprint profiles of Lonicerae japonicae Flos and Lonicerae Flos

作　　者：付珊珊[1,2] 张甜甜[1,2] 吕俊兰[1,2] 郭静静[1,2] 袁海龙[1] 肖小河[1] 

机构地区：[1]解放军第三0二医院,北京100039 [2]成都中医药大学,四川成都610075

出　　处：《药学学报》2011年第10期1251-1256,共6页Acta Pharmaceutica Sinica

基　　金：Project supported by the National Key New Drugs Innovation Foundation(2009ZX09502-022)

摘　　要：采用微量量热法从生物热活性角度,对金银花与山银花的生物指纹图谱进行比较研究。本文以山银花中的灰毡毛忍冬为例,考察不同浓度的金银花与灰毡毛忍冬提取液对志贺痢疾杆菌的抑制作用,测定其产热变化规律。以产热曲线和热动力学参数为评价指标,客观地表征金银花与灰毡毛忍冬对志贺痢疾杆菌生长代谢的影响,并对实验结果做了相似性分析。结果显示,金银花与灰毡毛忍冬有很强的抑制志贺痢疾杆菌生长代谢的作用且两者有很好的相似性。生物评价应与化学分析法相结合来控制中药质量,来共同确保中药的安全和疗效。To compare the microcalorimetric fingerprint profiles of Lonicerae japonicae Flos（Lj.F） and Lonicerae Flos（L.F）,microcalormietry was applied to find the heat change regularity of Bacillus shigae（B.shigae） metabolism affected by Lj.F and L.F（we choose Lonicera macranthoides Hand.– Mazz in this paper） with different concentrations.The thermogenic curves and thermodynamics parameters were investigated as evaluation index,and then the date of experiment was studied by similarity analysis.All the results indicated that the Lj.F and Lonicera macranthoides Hand.– Mazz（L.m.H-M） significantly impacted the microbial growth and had good similarity in its inhibitory activities.The combination approach of chemical analysis with bioassay was developed and employed to ensure the safety and efficacy of Chinese herbal medicines.

关 键 词：金银花 山银花 微量量热法 大肠杆菌 

分 类 号：R917[医药卫生—药物分析学]