葡萄籽原花青素对复发性结肠炎大鼠结肠组织中c-Jun、c-Fos表达的影响  

Effect of proanthocyanidins from grape seeds on c-Jun and c-Fos expression in colon tissues of recurrent colitis rats

作　　者：王艳红[1,2] 丁义兰[3] 杨孝来[2] 葛斌[2] 路莉[1] 张宝来[1] 於洪建[4] 刘丹[4] 吴勇杰[1] 

机构地区：[1]兰州大学基础医学院药理学研究所 [2]甘肃省人民医院药剂科 [3]兰州市妇幼保健院 [4]天津市尖峰天然产物研究开发有限公司,天津300000

出　　处：《中国临床药理学与治疗学》2011年第9期992-998,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：甘肃省自然科学基金资助项目(3ZS061-A25-083);国家自然基金资助项目(30672490)

摘　　要：目的:观察葡萄籽原花青素(GSPE)对复发性结肠炎大鼠结肠组织中c-Jun、c-Fos表达的影响,探讨GSPE治疗复发性结肠炎的分子作用机制。方法:雄性Wistar大鼠按体重随机分为正常对照组、模型对照组、柳氮磺吡啶(SASP)组和GSPE低(100 mg/kg)、中(200 mg/kg)、高(400 mg/kg)剂量组。用TNBS-50%乙醇二次致炎法建立大鼠溃疡性结肠炎(UC)复发的模型,于第2次致炎24 h后灌胃对其进行治疗,连续给药7 d后处死大鼠。取结肠标本进行大体与组织病理学损伤评分;用邻联茴香胺法检测结肠组织中髓过氧化物酶(MPO)活力以考察中性粒细胞浸润程度;应用酶联免疫法检测大鼠结肠组织中c-Jun与c-Fos的表达。结果:与正常对照组比较,模型对照组大鼠大体和组织病理学损伤评分及组织中MPO活力显著增加,结肠组织中c-Jun与c-Fos表达明显升高。给予不同剂量的GSPE治疗7 d后,明显减轻复发性结肠炎大鼠大体和组织病理学损伤评分,减轻肠黏膜中性粒细胞的浸润程度,抑制结肠组织c-Jun与c-Fos的高表达。而柳氮磺吡啶组大鼠肠组织中c-Jun与c-Fos含量与模型对照组比较无明显差异(P>0.05)。结论:GSPE可以抑制复发性结肠炎大鼠结肠组织中c-Jun与c-Fos蛋白的高表达。AIM： To investigate the effect of proanthocyanidins from grape seeds（GSPE） on c-Jun and c-Fos expression in colon tissues of recurrent colitis rats.METHODS： Wistar male METHODS： Wistar male rats were randomized divided into six groups： normal control group,model control group,Sulfasalazine（SASP） group,and the 3 GSPE treatment groups.Rats were intragastrically administered different doses of GSPE（100,200,and 400 mg/kg） per day for 7 days after recurrent colitis was twice-induced by TNBS-50% ethanol.Rats were killed after GSPE treatment 7 days.The colonic injury and inflammation were assessed by macroscopic and microscopic damage scores and myeloperoxidase（MPO） activity.The expression levels of c-Jun and c-Fos in the colon tissues were measured by enzyme-linked immunosorbent assay methods.RESULTS：Compared with the model group,the macroscopic and microscopic damage scores and the activity of MPO were significantly decreased in GSPE dosage group.Moreover,the expression levels of c-Jun and c-Fos in the colon tissues were significantly decreased.CONCLUSION： GSPE can inhibit the expression of c-Jun and c-Fos in the colon tissues of the recurrent colitis rats.

关 键 词：葡萄籽原花青素 三硝基苯磺酸(TN-BS) 结肠炎 复发 C-JUN c-Fos 

分 类 号：R965.2[医药卫生—药理学]