山奈酚诱导人小细胞肺癌H446细胞凋亡及机制  

Kaempferol-induced apoptosis in human small cell lung cancer H446 cells

作　　者：仇炜[1] 赵娟[2] 吕雨虹[2] 赵俊霞[2] 王彦玲[2] 雷宇华[2] 

机构地区：[1]北京大学医学部,北京100191 [2]河北医科大学细胞生物学教研室,河北石家庄050017

出　　处：《中国药理学通报》2011年第10期1422-1425,共4页Chinese Pharmacological Bulletin

基　　金：河北省科技支撑计划项目(No08206117D);河北省医学科学研究重点课题计划指导项目(No08259)

摘　　要：目的探讨山奈酚对人小细胞肺癌H446细胞的抑制作用,并研究其作用机制。方法采用MTT法、DAPI染色、流式细胞术等方法检测山奈酚对H446细胞增殖及凋亡的影响;使用Western blot检测山奈酚处理后H446细胞中p53、bax和bcl-2的表达变化。结果山奈酚抑制人小细胞肺癌H446细胞增殖,促进H446细胞阻滞于S期及G2/M期,诱导该细胞株凋亡,上调p53、bax的表达水平,降低bcl-2的表达水平。结论山奈酚对H446细胞有明显的抑制作用,该抑制作用与山奈酚诱导的细胞周期阻滞和细胞凋亡有关。Aim To investigate the inhibitory effects of kaempferol on human small cell lung cancer H446 cells.Methods MTT assays,DAPI staining and flow cytometry（FCM） were performed to investigate the effects of kaempferol on proliferation and apoptosis of H446 cells.Western blot assays were performed to analyze the expression of p53,bax and bcl-2.Results MTT assays suggested that kaempferol significantly inhibited proliferation.FCM assays demonstrated kaempferol induced S and G2/M phase cell cycle arrest to H446 cells.Furthermore,the administration of kaempferol to cultured H446 cells dramatically induced cell apoptosis.Western blot indicated that kaempferol up-regulated the expression of p53 and bax,but down-regulated the expression of bcl-2 in H446 cells.Conclusion Kaempferol inhibits H446 cells via inducing S and G2/M phase cell cycle arrest and apoptosis.

关 键 词：山奈酚 小细胞肺癌 凋亡 细胞周期 增殖 BAX BCL-2 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]