左旋精氨酸对气道上皮细胞白细胞介素6和8的调节作用  

Regulatory effects of L-arginine on interleukin-6 and interleukin-8 in airway epithelial cell line

作　　者：范晓云[1,2] 汪浩[1,2] 武晓兰[1,2] 汪伟民[1,2] 

机构地区：[1]安徽医科大学第一附属医院老年呼吸内科 [2]安徽医科大学呼吸病研究所,安徽合肥230022

出　　处：《中国新药与临床杂志》2011年第9期676-680,共5页Chinese Journal of New Drugs and Clinical Remedies

基　　金：国家自然科学青年基金项目(81100027);安徽省教育厅自然科学研究项目(KJ2011A176);安徽医科大学第一附属医院国家青年基金培育计划项目(2010KJ13)

摘　　要：目的研究左旋精氨酸对NCI-H292细胞中白细胞介素6(IL-6)和IL-8的影响。方法细胞共分为5组:最低需要培养基(MEM)组、MEM+A组(MEM+左旋精氨酸)、RPMI组(RPMI1640培养基,含1 mmol.L-1精氨酸)、常规培养基组(精氨酸含量>1 mmol.L-1)、地塞米松组(MEM+10μg.L-1地塞米松)。通过TNF-α和脂多糖刺激NCI-H292细胞建立哮喘气道炎症微环境,观察不同浓度TNF-α和脂多糖对IL-6和IL-8释放的影响,并比较各组间IL-6和IL-8水平。采用ELISA法测定细胞IL-6和IL-8释放水平,斑点印迹法测定其mRNA水平。结果 IL-6和IL-8水平与TNF-α的浓度正相关(r=0.644、0.715,均P<0.01)。不论有无脂多糖或TNF-α的刺激,MEM组IL-6和IL-8水平高于MEM+A组、RPMI组和地塞米松组(P<0.01)。MEM组IL-6水平高于常规培养基组(P<0.01),TNF-α刺激下IL-8水平高于常规培养基组(P<0.01),脂多糖刺激下2组IL-8水平相当(P>0.05)。地塞米松组IL-6和IL-8水平与MEM+A组相当(P>0.05)。细胞培养4、8、24 h,MEM+A组IL-6 mRNA和IL-8 mRNA表达显著低于MEM组(P<0.05)。结论一定浓度左旋精氨酸可抑制NCI-H292细胞IL-6和IL-8的释放,可能通过降低IL-6和IL-8转录活性引起,在哮喘治疗中具有潜在的应用价值。AIM To investigate the effects of L-arginine （Arg） on the production of interleukin-6 （IL- 6） and interleukin-8 （IL-8） in NCI-H292 cells. METHODS NCI-H292 ceils were divided into MEM group, MEM ＋ A group （MEM ＋ Arg）, RPMI group （RPMI1640 medium containing 1 mmol-L-1 Arg）, regular medium group （Arg 〉 1 mmol.L-1） and dexamethasone group （MEM ＋ 10 μg-L-1 dexamethasone）. NCI-H292 cells were stimulated with tumor necrosis factor （TNF-α） or lipopolysaccharide （LPS）, and the production of IL-6 and IL-8 induced by different concentrations of TNF-α or LPS in each groups were observed. The expressions of IL-6 and IL-8 were determined by ELISA, and the levels of mRNA were detected by dot blotting. RESULTS There was positive correlation between TNF-α and IL-6 （r = 0.644, P 〈 0.01）, IL-8 （r = 0.715, P 〈 0.01 ）, respectively. With or without LPS or TNF-α stimulation, the levels of IL-6 and IL-8 in the MEM group were much higher than those in the MEM ＋ A group, RPMI group and dexamethasone group （P 〈 0.01 ）. The level of IL-6 in the MEM group was higher than that in the regular medium group （P 〈 0.01 ）. The level of IL-8 in the MEM group was higher than that in the regular medium group （P 〈 0.01 ） with TNF-α stimulation, but no significance difference when stimulated by LPS （P 〉 0.05）. There were no significant differences in IL-6 and IL-8 between the MEM ＋ A group and dexamethasone group （P 〉 0.05）. At 4, 8 and 24 h of incubation, the mRNA expressions of IL-6 and IL-8 in the MEM ＋ A group were lower than those in the MEM group （P 〈 0.05）. CONCLUSION A certain concentration of Arg can inhibit the release of IL-6 and IL-8 in NCI-H292 cells by decreasing the transcriptional activity of IL-6 and IL-8.

关 键 词：哮喘 精氨酸 白细胞介素6 白细胞介素8 一氧化氮合酶 

分 类 号：R977.4[医药卫生—药品]