海水浸泡对大鼠深Ⅱ度烫伤后创面愈合的影响  

Effects of seawater immersion on wound healing following Ⅱ -degree scald in rats

作　　者：范子锋 尹秀珍 王甲汉[2] 吴永恒[2] 李志清[2] 易朝辉[2] 

机构地区：[1]解放军91676部队医院,广东省东莞523900 [2]南方医科大学南方医院烧伤科

出　　处：《中华航海医学与高气压医学杂志》2011年第4期221-223,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的 从创面炎症反应及感染等方面探讨海水浸泡对烫伤大鼠创面愈合的影响。方法 建立Wistar大鼠10％体表面积深Ⅱ度烫伤合并海水浸泡模型，采用数字表法，将120只雄性Wistar大鼠随机分为单纯烧伤对照组和海水浸泡实验组，实验组大鼠烫伤后立即用海水浸泡4h。观察组织病理改变、创面愈合时间和创面感染情况；ELISA法检测血清中TNF-α及IL-6含量。结果 实验组大鼠血清中TNF-α[（ 130.4±17.3） pg/ml]、IL-6[（320.5±28.5） pg/ml]含量与对照组比较，差异有统计学意义（P＜0.05）；创面感染加重，创面愈合时间延长（P＜0.05），再上皮化和表皮各层的分化延迟，且海水浸泡可使烧伤创面肿胀、创面局部组织炎症反应加重。结论 大鼠烫伤后合并海水浸泡，可加重创面炎症反应及创面感染，抑制创面愈合。Objective To investigate the effects of seawater immersion on wound inflammation and healing following Ⅱ -degree scald in rats. Methods One hundred and twenty male Wistar rats with 10% total body surface Ⅱ -degree scald were randomly divided into the scald group （group A） and the scald plus seawater immersion group （ group B）. Rats in group B were immersed in seawater for 4 hours, soon after they were scalded. Wound infection, the time of healing and changes in histopathology were closely observed. Levels of serum interleukin- 6 （ IL- 6 ） and tumor necrosis factor （ TNF- α ） were determined with enzyme-linked immunosorbent assay （ELISA）. Results Levels of serum IL- 6 （320. 5 ± 28.5 ） pg/ml and TNF-α content （ 130.4 ± 17.3 ） pg/ml in group B increased significantly, when they were compared with those of group A （P 〈0. 05 ）. Moreover, wound surface was seriously infected and wound healing time prolonged （P 〈 0. 05 ）. Biological culture showed that seawater immersion could promote bacteria growth. Pathological examination indicated that seawater immersion could aggravate wound swelling and inflammatory response of the injured tissue, and inhibit epithelialization and differentiation of various epidermal layers. Conclusions Scald coupled with seawater immersion could aggravate inflammatory response of the wound surface and inhibit wound healing in rats.

关 键 词：烧伤 海水浸泡 肿瘤坏死因子 白介素-6 创面愈合 

分 类 号：R285.5[医药卫生—中药学]