附片水煎制剂中乌头类生物碱水解物含量测定研究  

Study on Hydrolyzate from Aconitine-type Alkaloids in Monkshoo Decoction of Radix Aconiti

作　　者：陈玉[1] 郭平[2] 伍丽萍[3] 陈聪[2] 费小凡[1] 叶利明[2] 

机构地区：[1]四川大学华西医院药剂科,成都610041 [2]四川大学华西药学院,成都610041 [3]成都市妇幼保健院

出　　处：《华西医学》2011年第9期1382-1385,共4页West China Medical Journal

摘　　要：目的考察用乌头碱水解物、新乌头碱水解物、次乌头碱水解物的含量作为附片水煎剂及附片质量控制指标的可行性。方法采用高效液相色谱分析法,色谱柱为Shimpack CLC-ODS,以甲醇-乙酸铵溶液(0.2 mol/L)(200?210)为流动相,流速1.0 mL/min,检测波长为241 nm,对乌头碱新,乌头碱、次乌头碱水解物的含量测定进行研究。结果 3种水解物检测方法测得水解物在进样范围有良好线性关系,重现性好,稳定性好,生药提取时间宜选定为40 min,不同批号的附片中3种水解物含量差别较大。结论此方法简便,使用新乌头碱、乌头碱、次乌头碱的水解物作为含附片制剂的质量控制指标是可行的。Objective To examine the feasibility of regarding the hydrolyzate from aconitine, new aconitine, and hypaconitine to be the decoction of radix aconite and the quality control index. Methods High performance liquid chromatographic method （HPLC） method was adopted. Shimpaek CLC-ODS column with UV detection at 241 nm was used. The mobile phase consisted of （200 ： 210）, and the flow rate was 1.0 mL/min. Results The content of the hydrolysis objects in the water decoction and toxicity were correlated with each other, and the contents of hydrolysis were significantly different among different groups of radix aconiti. Conclusion This method is simple, accurate and effective for content research of hydrolyzate from aconitine-type alkaloids in Monkshoo decoction of Radix Aconiti, and it is feasible to take it as the quality control index.

关 键 词：高效液相色谱法 乌头碱类生物碱水解物含量测定 质量控制 

分 类 号：R284.1[医药卫生—中药学]