高效液相色谱法检测肾病综合征患者泼尼松血药浓度及其临床意义  

HPLC Determination of Prednisone in Serum of Nephrotic Syndrome and its Clinical Significance

作　　者：夏运成[1] 张旭光[2] 彭灿辉[1] 李铮[1] 彭佑铭[1] 

机构地区：[1]中南大学湘雅二医院肾病研究所,长沙410011 [2]潍坊医学院附属医院检验科,潍坊261031

出　　处：《中国中西医结合肾病杂志》2011年第9期758-761,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：湖南省自然科学基金资助项目(No.06JJ4020)

摘　　要：目的:建立一种高效液相色谱(HPLC)法测定泼尼松(PS)血药浓度的方法,并探讨肾病综合征PS血药浓度与蛋白尿消减关系。方法:以乙酰苯胺为内标,采用HP1100系列高效液相色谱仪,分析柱HPLichrosphere Ca柱(250mm×4mm,5μm);流动相为:甲醇∶四氢呋喃∶水=25∶25∶50(V/V),流速为1.0ml/min;检测波长为240nm;室温25℃。血清标本以甲醇沉淀蛋白。结果:泼尼松浓度与峰面积比值之间的线性关系良好,回归方程为Y=6.84×10-4×-39.4×10-4,相关系数为0.99993,回收率为98.70%,批间CV3.76%,批内CV2.25%。线性范围:10～500μg/ml;检测限:5μg/ml。NS组与对照组比较血液平均SP浓度,差异有统计学意义(P<0.05);男女组别比较,差异无统计学意义(P>0.05);激素抵抗组与激素敏感组血药浓度比较,抵抗组患者SP血药低于敏感组血药浓度,浓度差异有统计学意义(P<0.05)。结论:高效液相色谱法检测SP血药浓度,色谱峰分离良好,无干扰,是一种可靠、便捷的检测血清PS浓度的方法,对监测长期应用泼尼松治疗类疾病和肾病综合征的血药浓度监测(TDM),了解用药个体化差异,规范用药方法,为临床治疗提供科学、合理用药理论依据和重要参考,对药效学研究具有重要临床意义。Objective：To develop a high-performance liquid chromatography method to determine the concentration of prednisone（PS）in human serum,and to assess the relationship between serum concentration of PS and changes in urinary protein.Methods：The HP1100 high performance liquid chromato-graphic（HPLC）system was used.The analytic column was a HP Lichrosphere C8 column（250 mm×4 mm,5 μm）.The mobile phase was methanol,tetrahydrofuran and water with the ratio 25∶25∶50 The flow rate was 1.0 ml/min The detective UV wavement was 240 nm The serum samples,added acetanilide as internal standard,deposited down the protein with methanol.Results：The chromatography was good and not interfered by the components of the serum.Good Linearity was observed in 10～500 μg/L range for prednisone,the detection limit was 5 mg/ml.The serum concentration of NS group was different from that in the control group（P0.05）,there was no significant difference between the females and males groups（P0.05）,the serum concentration of hormone resistant group was lower significantly compared with hormone sensitive group（P0.05）.Conclusion：This is an accurate and convenient method to determine the concentration of PS in serum,it can monitor the plasma concentrations of PS in patients with long-term PS treatment and nephrotic syndrome,and help to know Individualized differences,normalize the preparation method,It holds significant implications in the Pharmacodynamic Study.

关 键 词：高效液相色谱 泼尼松 血药浓度监测 临床意义 

分 类 号：R692.3[医药卫生—泌尿科学]