聚乙二醇化干扰素α-2a对慢性乙型肝炎患者外周血T淋巴细胞亚群的影响  

The effect of PGE-interferon-alpha2a on peripheral T lymphocyte subsets in Patients with chronic hepatitis B

作　　者：阚晓[1] 隋赟[2] 韩绍磊[1] 曹创杰[1] 郑昭敏[1] 

机构地区：[1]济南市传染病医院,山东济南250021 [2]济南市第二人民医院,山东济南250021

出　　处：《基础医学与临床》2011年第10期1139-1143,共5页Basic and Clinical Medicine

摘　　要：目的动态观察慢性乙型肝炎患者(CHB)聚乙二醇化干扰素α-2a治疗过程中外周血T淋巴细胞亚群的变化及其临床意义。方法给予52例CHB患者聚乙二醇化干扰素α-2a(PEG-IFNα-2a)抗病毒治疗48周。在治疗前、治疗12和48周,分别以流式细胞术检测外周血淋巴细胞亚群百分比和数量,全自动生化分析仪检测谷丙转氨酶(ALT)水平,荧光定量PCR检测血清HBV DNA水平,酶联免疫吸附法检测HBV标志物。结果 52例CHB患者CD4+、CD4+/CD8+比值均下降(P<0.05),CD8+T升高(P<0.05)。PEG-IFNα-2a抗病毒治疗过程中,患者外周血CD4+T、CD4+/CD8+比值明显升高(P<0.05),CD8+T值下降(P<0.01),48周时基本恢复至正常水平。治疗12和48周时,HBeAg阴转率分别为44.2%和51.9%,其中11例(21.1%)发生血清学转换者在治疗12周时CD4+、CD4+/CD8+比值均已恢复至正常人水平。结论 PEG-IFNα-2a治疗慢性乙型肝炎致患者CD4+、CD4+/CD8+比值升高可获得持续性病毒学及生化学应答的概率高,抗病毒效果好。Objective To study the change of peripheral T lymphocyte subsets and its impacton patients with chronic hepatitis B（CHB） during peginterferon-alph2a treatment.Methods Fifty-two patients with chronic hepatitis B were recruited and received peginterferon-alph2a treatment for 48 weeks.Before and during the 12 and 48 weeks of treatment,flow cytometry was used to detect the Peripheral T lymphocyte subsets.Realtime PCR was used to detect the level of HBV DNA.Markers of hepatitis B virus infection were detected by ELISA assay and level of alanine aminotransferase in the serum was measured by automatic biochemical analyzer.Results The proportion of peripheral blood CD4＋、CD4＋ / CD8＋ in patients with CHB was significantly lower than that in healthy people（P0.05） and the proportion of peripheral blood CD8＋T lymphocytes are increasing（P0.05）.During 12 weeks of INFa treatment the proportion of peripheral blood CD4＋T lymphocytes and the ratio of CD4＋ / CD8＋ increased gradually,the proportion of peripheral blood CD8＋T lymphocytes decreased to normal level after 48 weeks INFα treatment.Treatment for 12 and 48 weeks with INFα resulted in HBeAg seroconversion to negative in 44.2% patients and 51.9% patients respectively.In 11（21.1%） patients who had seroconvertion from HBeAg to anti-HBeAg,after 12 months of INFa treatment,their proportion of peripheral blood CD4＋、CD4＋ / CD8＋ had increased to level similar to that of the healthy people.Conclusions INFa treatment reduces HBV replication and increases the proportion of peripheral blood CD4＋、CD4＋ / CD8＋ and these patients are prone to have higher sustain virus response and higher biochemical response.

关 键 词：慢性乙型肝炎 淋巴细胞亚群 PEG-IFNα-2a CD4+ CD8+ 

分 类 号：R512.62[医药卫生—内科学]