肠三叶因子和黏蛋白对烧伤血清所致肠上皮细胞增殖移行能力变化的影响  

Effects of intestinal trefoil factor combined with mucin on ability of proliferation and migration of intestinal epithelial cells after being treated by burn rat serum

作　　者：王焕[1] 吴修文[1] 万千雪[1] 金星[1] 孙勇[1] 吴丹[1] 曹俊杰[1] 彭曦[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所 创伤、烧伤与复合伤国家重点实验室,重庆400038

出　　处：《中华烧伤杂志》2011年第5期347-352,共6页Chinese Journal of Burns

基　　金：基金项目：国家自然科学基金（30971075）;“重大新药创制”科技重大专项（2009ZX09103-647）;创伤、烧伤与复合伤国家重点实验室自主研究课题（SKLZZ200807）

摘　　要：目的观察烧伤血清所致肠上皮细胞增殖、移行能力的变化，以及联合应用肠三叶因子（ITF）和黏蛋白对其的影响。方法将大鼠小肠上皮细胞株IEC-6传代培养，按照随机数字表法分为正常对照组、烧伤血清组、ITF＋烧伤血清组、黏蛋白＋烧伤血清组和ITF＋黏蛋白＋烧伤血清组，均采用DMEM培养液培养，其内分别添加体积分数10％小牛血清、体积分数10％烧伤大鼠血清、25ug／mLITF和体积分数10％烧伤大鼠血清、250ug／mL黏蛋白和体积分数10％烧伤大鼠血清以及联合使用上述剂量ITF、黏蛋白和烧伤大鼠血清。处理后0～4d观察细胞增殖能力；划痕实验后12、24、36、48、72h观察细胞移行情况；Transwell法（细胞置于上室、培养液加入下室）培养4、6、8、10、12h，观察细胞变形能力，以下室内细胞计数表示。对数据进行t检验。结果（1）细胞增殖能力。处理后1～4d烧伤血清组细胞数量明显低于正常对照组（t值为-16．569～-2．613，P〈0．05或P〈0．01）。ITF＋烧伤血清组和黏蛋白＋烧伤血清组细胞数量与烧伤血清组接近（t值分别为0．037～0．740、0．116～0．429，P值均大于0．05）；处理后2d，ITF＋黏蛋白＋烧伤血清组细胞数量明显高于烧伤血清组（t=6．484，P〈0．01）、ITF＋烧伤血清组（t=3．838，P〈0．01）。（2）细胞移行能力。烧伤血清组划痕后各时相点细胞移行距离均远远短于正常对照组（t值为-37．594～-6．727，P值均小于0．01）。与烧伤血清组比较，黏蛋白＋烧伤血清组划痕后各时相点细胞移行距离无明显变化（t值为0．055—0．589，P值均大于0．05）。ITF＋烧伤血清组划痕后12、24、36h细胞移行距离分别为（47±6）、（126±13）、（170±11）um，明显长于烧伤血清组[（42±7）、（98±14）、（154±22）um，t值为2．230～4．817，P〈0．05或P〈0．01]。ITF＋黏蛋白＋烧伤�Objective To observe the effect of intestinal trefoil factor （ITF） combined with mucin on the ability of proliferation and migration of intestinal epithelial cells （IEC） after being treated by burn rat serum. Methods The rat IEC-6 cell lines were subcultured and divided into control group （C, cultured with DMEM medium containing 10% calf serum） , burn serum group （ BS, cultured with DMEM medium containing 10% burn rat serum） , burn serum ＋ ITF group （B ＋ I, cultured with DMEM medium containing 10% burn rat serum and 25 ug/mL ITF） , burn serum ＋ mucin group （ B ＋ M, cultured with DMEM medium containing 10% burn rat serum and 250 ug/mL mucin） , and burn serum ＋ ITF ＋ mucin group （ B ＋ I ＋ M, cultured with DMEM medium containing 10% burn rat serum, 25 ug/mL ITF, and 250 ug/mL mucin） according to the random number table. Cells were counted on post culture day （PCD） 0, 1, 2, 3, 4, reflecting cell proliferation ability. Cell migration distance was measured at post scratch hour （PSH） 12, 24, 36, 48, 72. Then, cells of each group were placed in upper compartment of Transwell chamber while the corresponding medium was respectively added into lower compartment of Transwell chamber. Cells in lower com- partment of Transwell chamber were counted at post culture hour （PCH） 4, 6, 8, 10, 12, reflecting cytomorphosis ability. Data were processed with t test. Results （1） Cell proliferation ability. The cell numbers in BS group on PCD 0, 1, 2, 3, 4 were significantly less than those in C group （with t values from - 16. 569 to - 2. 613, P 〈 0.05 or P 〈 0.01 ）. The cell number showed no statistical difference between B ＋ I and BS groups, and between B ＋ M and BS groups at each time point （ with t values respectively from 0.037 to0.740 and 0. 116 to 0.429, Pvalues all above 0.05）. The cell number in B ＋I ＋M group on PCD 2 was respectively larger than that in BS group （ t =6.484, P 〈0.01） and B＋I group （ t =3.838, P 〈0.01）. （2） Cell migr

关 键 词：烧伤 黏蛋白类 细胞增殖 细胞移行 肠三叶因子 肠上皮细胞 

分 类 号：R644[医药卫生—外科学]