束缚-浸水应激对大鼠疑核胆碱能及儿茶酚胺能神经元活动的影响  

Influence of restraint water-immersion stress on activities of cholinergic and catecholaminergic neurons in the nucleus ambiguous in rats

作　　者：赵东芹[1] 艾洪滨[1] 

机构地区：[1]山东师范大学生命科学学院生理学教研室,济南250014

出　　处：《山东大学学报（医学版）》2011年第10期103-106,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30770277;30970354;31071920)

摘　　要：目的研究束缚-浸水应激(RWIS)大鼠疑核(NA)中胆碱能、儿茶酚胺能神经元的活动情况。方法随机将10只雄性Wistar大鼠分为对照组和应激组(RWIS组),采用Fos、胆碱乙酰转移酶(ChAT)或酪氨酸羟化酶(TH)免疫组织化学双标技术,统计对照组和应激组Fos、ChAT或TH单标阳性神经元以及Fos+ChATT或Fos+TH双标阳性神经元数目。结果 NA中ChAT免疫阳性神经元胞体呈梭形或多角形,有较多的TH阳性终末。与对照组相比,RWIS组大鼠NA大量神经元表达Fos(P<0.05);RWIS组Fos+ChAT双标阳性神经元数目也较对照组显著增加(P<0.05),占ChAT免疫阳性神经元的比例在对照组和RMS组中分别为7.5%、29.8%(P<0.01)。结论 NA中胆碱能神经元参与RWIS调控过程,而这些神经元可能接受其他核团儿茶酚胺能神经元的纤维投射。Objective To investigate activities of cholinergic and catecholaminergic neurons in the nucleus ambiguous （NA） in response to water-immersion stress（RWIS） in rats.Methods Ten rats were randomly divided into the control group and the RWIS group.Fos-immunoreactive（Fos-IR）,choline acetyltransferase immunoreactive（ChAT-IR）, tyrosine hydroxylase immunoreactive（TH-IR） and double labeled（Fos ＋ ChAT-IR and Fos ＋ TH-IR） neurons were counted using immunohistochemistry.Results In the NA,ChAT-IR neurons were fusiform or polygonal,with a large amount of TH-IR ending.Compared with unstressed rats,the number of Fos-IR neurons was dramatically increased in the NA of RWIS rats（P0.05）,and the number of Fos ＋ ChAT-IR neurons was greater in RWIS rats than unstressed rats（P0.05）.The percentage of Fos-IR nuclei in ChAT-IR neurons in unstressed and RWIS rats were 7.5%and 29.8%,respectively（P0.01）.Conclusion Cholinergic neurons in the NA,which might be regulated by catecholaminergic neurons in other nuclei,are involved in the RWIS response.

关 键 词：应激 疑核 胆碱0-乙酰转移酶 酪氨酸羟化酶 FOS 

分 类 号：Q42[生物学—神经生物学]