Bt-BADH-GA20ox-rolB多基因在转化烟草中的表达分析  

Expression analysis of Bt-BADH-GA20ox-rolB multigenes in transgenic tobacco plants

作　　者：孙立洋[1] 李静[1] 袁虎威[1] 姚景涵[1] 李伟[1] 陈晓阳[2] 

机构地区：[1]北京林业大学林木育种国家工程实验室 [2]华南农业大学林学院

出　　处：《北京林业大学学报》2011年第5期86-90,共5页Journal of Beijing Forestry University

基　　金：教育部科学技术研究重点项目(01028)

摘　　要：为探讨Bt-BADH-GA20ox-rolB多基因转化烟草中外源基因的表达情况及对转化植株的影响,对转多基因烟草及对照植株进行了生理生化试验,包括内源激素含量测定、耐盐性试验、Bt毒蛋白测定、移栽后植株生长的观测。结果表明:转基因植株生根能力强,根、茎、叶各部位内源激素(IAA、GA3)与对照差异明显;在低浓度NaCl下,转基因和对照植株生长差异不明显,当NaCl浓度达到300mmol/L时,对照生长受到抑制,转基因植株生长正常;在转基因植株的茎、叶中均检测到高浓度的Bt毒蛋白,以叶片饲喂斜纹夜蛾幼虫明显抑制其发育。移栽后的转基因植株高生长更加明显,苗高和地茎分别为对照的1.61和1.37倍。In order to discuss the exogenous genes expression and effect of Bt-BADH-GA20ox-rolB multigenes on transgenic plants,four transgenic tobacco lines and a control strain were taken as materials and the experiments included determination of endogenous hormones,salt tolerance test,Bt toxic protein measurement and growth observation.The results indicated that the rooting ability of transgenic plants was enhanced.Endogenous hormones（IAA,GA3）derived from roots,stems and leaves were significantly different from controls.At a low concentration of NaCl,there is no difference between transgenic plant and control,while at 300 mmol/L of NaCl the growth of control was restrained with and the transgenic plant was normal.The Bt toxic protein was detected at a high level in both stems and leaves of the transgenic plants.The development of prodenia was obviously inhibited by feeding the leaves of transgenic plants.The transgenic plants grew vigorously after being transplanted.The average height and basal stem of transgenic plants were 1.61 and 1.37 times higher than those of control respectively.

关 键 词：Bt-BADH-GA20ox-rolB多基因 内源激素 耐盐性 BT毒蛋白 

分 类 号：Q943.2[生物学—植物学] Q945