广州市甲型H1N1流感暴发疫点与监测人群病毒抗体检测  

Serological analysis of antibodies to influenza A(H1N1) viruses in an outbreak area in Guangzhou

作　　者：鲁恩洁[1] 周颖[1] 陈艺韵[1] 吴继彬[1] 李铁钢[1] 许杨[1] 耿进妹[1] 谢华萍[1] 蒋力云[1] 吴新伟[1] 伍业健[1] 李魁彪[1] 狄飚[1] 王鸣[1] 

机构地区：[1]广州市疾病预防控制中心,广东广州510440

出　　处：《热带医学杂志》2011年第9期1073-1075,共3页Journal of Tropical Medicine

基　　金：广州市科技计划项目(2008YB-132)

摘　　要：目的通过分析广州市甲型H1N1流感暴发疫点与监测人群病毒的抗体水平,了解甲型H1N1流感的流行趋势,为预防甲型H1N1流感疫情提供科学依据。方法应用红细胞血凝抑制(HI)方法检测流感甲型H1N1抗体,对比分析1570名疫区学生与1326名监测人群血清标本H1N1流感病毒的抗体水平。结果疫区学生甲型H1N1流感病毒感染率、流感罹患率分别为32.17%、22.23%,明显高于市区监测人群的22.62%、15.38%(P=0.000,P=0.000)。疫点学生与市区监测人群甲型H1N1流感隐性感染率分别为9.94%、7.24%,差异有统计学意义(P=0.012)。在已感染甲型H1N1流感病毒的疫点学生和市区监测人群中,甲型H1N1流感隐性感染率(30.89%、32.00%)差异无统计学意义(P=0.754)。疫点人群显性感染者的抗体滴度明显高于隐性感染者(t=4.701,P=0.000),监测人群中显性感染与隐性感染者的抗体滴度无显著差异(t=0.248,P=0.804)。结论疫点学生甲型H1N1流感隐性感染率明显高于监测人群。提示隐性感染人群具有潜在的传染力,应加强隐性感染者的监测。Objective To explore the epidemic trend of influenza A（H1N1） viruses outbreak through the investigation of antibodies of the influenza A（H1N1） viruses in the flu outbreak areas in Guangzhou.Methods Serum samples from 1 570 students living in the outbreak areas and 1 326 subjects living in the surveillance area were collected.The titers of antibodies to the influenza A（H1N1） viruses were detected by hemagglutination-inhition test.Results The infection rates and the incidence of influenza A（H1N1） infection were 32.17% and 22.23%,respectively,for the students living in the outbreak areas,compared to 22.62% and 15.38% in the surveillance populations.There were significant difference in the infection rates（P=0.000） and the incidence of influenza A（H1N1） infection（P=0.000） between the two groups.The rate of subclinical infection were respectively 9.94%（students in outbreak area） and 7.24%（surveillance populations）（P=0.012）.There was no significant difference in the rate of subclinical infection between the two groups.The level of antibody of overt infections to influenza A（H1N1） was significantly higher than the recessive infections in the outbreak area students（t=4.701,P=0.000）.There was no significant different in the level of antibody between clinical infections and subclinical infections in the surveillance populations（t=0.248,P=0.804）.Conclusion The rate of subclinical infection to influenza A（H1N1） in the outbreak area students was higher than the surveillance populations.These results suggest that individuals with subclinical infection to influenza A（H1N1） may be the reservoir for this virus.It is important for the disease control to strengthen the viral surveillance and provide the preventive education in middle school during the period of H1N1 influenza pandemic.

关 键 词：甲型H1N1流感 抗体滴度 血凝抑制 

分 类 号：R373.13[医药卫生—病原生物学]