Enhanced Immunostimulatory Properties in CpG-Oligonucleotides Modified by Gold Nanoparticles  

作　　者：周寅 张红菱 

机构地区：[1]College of Biology & Pharmacy Engineering,Wuhan Polytechnic University

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2011年第5期832-836,共5页武汉理工大学学报（材料科学英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.20807017);Doctoral Fund of Ministry of Education of China (No.20080487087);the Fundamental Research Funds for the Central Universities (No.2010MS091)

摘　　要：Whether the immunostimulatory effects of CpG-oligonucleotides （CpG ODNs） could be enhanced by the use of gold nanoparticles （Au-NP） was investigated.The CpG ODNs were modified by the Au-NP （CpG /Au-NP） and their uptake and distribution in murine N9 microglial cells were studied.The immunostimulatory effects of CpG /Au-NP on N9 cells,human B cells and plamacytoid dendritic cells （pDCs） were examined.Results showed that the uptake of CpG /Au-NP in N9 cells was much higher than that of CpG ODNs and CpG /Au-NP localized in the cytoplasm of N9 cells.The amount of TNF-α and IL12p40 in N9 cells was increased greatly by the use of Au-NP.And the amount of IL-6 in B cells and IFN-α in pDCs was also significantly increased,while the activation of B cells was not changed.These results reveal that the Au-NP can be used as a delivery media for the oligonucleotides-based therapeutics.Whether the immunostimulatory effects of CpG-oligonucleotides （CpG ODNs） could be enhanced by the use of gold nanoparticles （Au-NP） was investigated.The CpG ODNs were modified by the Au-NP （CpG /Au-NP） and their uptake and distribution in murine N9 microglial cells were studied.The immunostimulatory effects of CpG /Au-NP on N9 cells,human B cells and plamacytoid dendritic cells （pDCs） were examined.Results showed that the uptake of CpG /Au-NP in N9 cells was much higher than that of CpG ODNs and CpG /Au-NP localized in the cytoplasm of N9 cells.The amount of TNF-α and IL12p40 in N9 cells was increased greatly by the use of Au-NP.And the amount of IL-6 in B cells and IFN-α in pDCs was also significantly increased,while the activation of B cells was not changed.These results reveal that the Au-NP can be used as a delivery media for the oligonucleotides-based therapeutics.

关 键 词：Au nanoparticles modified CpG-oligonucleotides delivery media immunostimulatory properties 

分 类 号：TB383.1[一般工业技术—材料科学与工程]