染色法鉴别旋毛形线虫成虫死活的实验观察  

Experimental study on determination of viability of the adult of Trichinella spiralis in vitro

作　　者：王小莉[1,2] 李亮[3] 方强[2] 崔洁[2] 郭凯[2] 沈继龙[1] 

机构地区：[1]安徽医科大学病原生物学教研室,安徽合肥230032 [2]蚌埠医学院病原生物学教研室,安徽省感染与免疫重点实验室,安徽蚌埠233030 [3]蚌埠学院食品与生物工程系,安徽蚌埠233000

出　　处：《蚌埠医学院学报》2011年第10期1041-1042,1046,共3页Journal of Bengbu Medical College

基　　金：国家重点基础研究发展计划(973计划)资助项目(2010CB530000)

摘　　要：目的:探讨快速鉴别旋毛形线虫成虫死活的染色方法。方法:分别以0.5%番红、1.0%中性红和0.5%甲基蓝3种染液,对死或活旋毛形线虫成虫进行染色。结果:0.5%番红、1.0%中性红染液能使死亡旋毛形线虫成虫着色;旋毛形线虫活成虫对3种染液均不着色。结论:0.5%番红、1.0%中性红染液染色均可快速鉴别旋毛形线虫成虫死活。Objective：To explore the prompt methods in determination of viability of the adult of Trichinella spiralis.Methods：The adult worms of Trichinella spiralis were stained with 0.5% safranine,1.0% neutral red or 0.5% methylene blue.Results：The dead adults were observed when stained with 0.5% safranine or 1.0% neutral red in the study.The vital adults were unstained with all three stains.Conclusions：Both 0.5% safranine staining and 1.0% neutral red staining are prompt method in determination of viability of the adult of Trichinella spiralis.

关 键 词：旋毛形线虫 成虫 番红 中性红 甲基蓝 

分 类 号：R383.15[医药卫生—医学寄生虫学]