合浦珠母贝C型凝集素2的序列特征和表达分析  

Sequence and Expression Analysis of a Novel C-type Lectin Gene from Pearl Oyster Pinctada fucata

作　　者：胡钰婷[1,2] 张殿昌[1] 江世贵[1] 苏天凤[1] 崔淑歌[1,3] 郭华阳[1,2] 陈明强[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广州510300 [2]上海海洋大学水产与生命学院,上海201306 [3]暨南大学生命科学技术学院,广州510632

出　　处：《湖北农业科学》2011年第19期4026-4030,共5页Hubei Agricultural Sciences

基　　金：公益性行业(农业)科研专项(200903028);国家863计划项目(2009AA10Z106);中央级公益性科研院所基本科研业务费专项(2010YD03);广东省科技计划项目(2010B020308001)

摘　　要：构建了合浦珠母贝(Pinctada fucata)的cDNA文库,并通过EST序列筛选和重新测序获得了一个新的C型凝集素基因的cDNA序列,命名为PoLEC2,其长度为1 659 bp,5’端非编码区为39 bp,3’端非编码区为45 bp,开放阅读框为1 575 bp,可编码524个氨基酸;PoLEC2蛋白的分子量约58.0 kD,等电点为5.2;PoLEC2蛋白包含一个信号肽序列(M1-A16)、一个MAM-2结构域和一个C型凝集素的糖识别结构域。同源性分析结果表明,PoLEC2与其他物种C型凝集素的糖识别结构域序列的同源性在26.5%～33.6%,相似性在45.1%～55.6%。组织表达分析表明,PoLEC2基因在肝胰脏、鳃、外套膜、性腺及肠组织中均有表达,经溶藻弧菌刺激后8h在肠组织中的表达量显著上调。The cDNA library was constructed from the pearl oyster （Pinctada fucata）. A C-type lectin gene, named as PoLEC2, was obtained by screening and sequencing using EST sequences. The length of PoLEC2 cDNA sequence was 1 659 bp. Sequence analysis showed that the open reading frame of PoLEC2 gene was 1575 hp. The 5＇-UTR length was 39 bp and 3＇-UTR length was 45 bp. The PoLEC2 gene encoded a protein containing 524 amino acids, and the predicted molecular weight of PoLEC2 protein was about 58.0 kD with the isoelectric point of 5.2. The protein sequence of PoLEC2 gene contained a signal peptide sequence with 16 amino acids （M^1-A^16）, a MAM-2 domain and a carbohydrate recognition domain of C-type lectin. Sequence analysis showed that the identity and similarity of the carbohydrate recognition domain of PoLEC2 genes between the P. fucata and other organisms were 26.5%-33.6% and 45.1%-55.6% respectively. Tissue expression analysis showed that the PoLEC2 gene was expressed in all tissues of P. fitcata, and there was maximum expression in the intestine after 8 hours of the bacterial infection.

关 键 词：合浦珠母贝(Pinctada fucata) C型凝集素 序列特征 表达分析 

分 类 号：S917.4[农业科学—水产科学]