授精激活介质对大眼鳜精子活力的影响  

Effects of Activating Media on the Sperm Motility of Siniperca kneri

作　　者：王芳[1] 岳兴建[1] 谢碧文[1] 齐泽民[1] 王淯[1] 葛正良 

机构地区：[1]内江师范学院生命科学学院/特色农业资源研究与利用四川省高校重点实验室,四川内江641112 [2]四川省资中县水产渔政局,四川资中641200

出　　处：《湖北农业科学》2011年第19期4031-4035,共5页Hubei Agricultural Sciences

基　　金：四川省教育厅重大培育项目(09ZZ012);四川省省属高校科研创新团队项目(KYTD201009);内江市科技局应用技术研究与开发项目(200901)

摘　　要：以不同pH溶液、不同浓度的葡萄糖和Na+、K+、Ca2+、Mg2+4种不同浓度金属离子及河水、曝气自来水、去离子水、蒸馏水4种水体作为授精激活介质,探讨大眼鳜(Siniperca kneri)精子在不同激活液中的活力变化。结果表明,pH为8.0时精子活力最强。大眼鳜精子的活力受葡萄糖、Na+、K+、Mg2+、Ca2+浓度的影响,葡萄糖浓度为1.5 g/L时精子活力最强,Na+、K+、Mg2+和Ca2+溶液浓度分别为75、0.9、15和10 mmol/L时精子活力最强。4种水体中精子活力由强到弱的顺序依次为蒸馏水、去离子水、曝气自来水和河水。The sperm motility of Siniperca kneri were investigated in 4 different solutions and of the river water, the tap water, the deionized water and distilled water, different solutions and waters, including different pH, different concentration of glucose, Na^＋,K^＋,Ca^2＋ and Mg^2＋. The results showed that the highest sperm motility was appeared in pH 8.0; The sperm motility of Siniperca kneri was affected by the glucose and the concentrations of Na^＋,K^＋,Mg^2＋,Ca^2＋, the highest sperm motility occurred when the concentration of glucose is 1.5 g/L; The highest sperm motility was appeared when the concentrations of Na^＋,K^＋,Mg^2＋,Ca^2＋ were 75,0.9,15 and 10 mmol/L respectively. The sperm motility in the distilled water, the deionized water, the tap water and the river water showed a trend from strong to weak.

关 键 词：大眼鳜(Siniperca kneri) 精子活力 PH 葡萄糖 金属离子 

分 类 号：S961.22[农业科学—水产养殖]