甘蓝型油菜及其亲本种BAC末端重复序列分析  

BAC End Repeat Sequence Analysis of Brassica napus and Its Parental Species

作　　者：曹睿[1] 黎瑞源[1] 龙艳[1] 孟金陵[1] 

机构地区：[1]华中农业大学植物科学技术学院,武汉430070

出　　处：《湖北农业科学》2011年第19期4073-4076,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30900788)

摘　　要：以芸薹属的3个物种(甘蓝型油菜Brassica napus、白菜B.rapa、甘蓝B.oleracea)的BAC末端序列为研究对象,对其重复序列的组成和比例、GC含量做了初步的比较研究。3个物种中BAC末端重复序列所占的比例分别是18.98%、19.37%和19.45%。3个物种的BAC末端重复序列均可分为反转座子、转座子、小RNA、微卫星、简单重复序列和低复杂度序列6种类型,其中反转座子所占比例最大,甘蓝型油菜rDNA的含量明显高于其他两个物种中该重复类型的含量。The repeat sequences＇ composition and proportion, GC content in BAC end sequences of Brassica species and its parental species Brassica napus, B. rapa, B. oleracea were comparatively analyzed. The proportion of BAC and repeat sequences in B. napus, B. rapa, B. oleracea was 18.98%, 19.37%, 19.45%, respectively. The repeat sequences in BAC end of the three species could be classified into 6 types of retrotransposon, transposon, miRNAs, microsatellite, simple sequence repeat and low complexity sequence, among which retrotransposon contributed to the highest proportion; and the ratio of rDNA in B. napus was much higher than that in the other two species.

关 键 词：甘蓝型油菜(Brassica napus) 亲本种 BAC末端 重复序列 

分 类 号：S565.4[农业科学—作物学]