感染A型产气荚膜梭菌小鼠体内α毒素的分布及定位  

Distribution and Localization of Aalpha Toxin in Mice Infected With Clostridium perfringens Type A

作　　者：王苗利[1] 蔡玉梅[1] 柴同杰[1] 张红娜[1] 

机构地区：[1]山东农业大学动物科技学院环境微生物实验室,山东泰安271000

出　　处：《动物医学进展》2011年第10期19-23,共5页Progress In Veterinary Medicine

基　　金：山东农业大学青年科技创新基金(23655);中国国际合作项目"动物疫源性人畜共患病的监测"(2009DFA32890)

摘　　要：为了研究α毒素在感染A型产气荚膜梭菌动物的体内的分布情况,自制了高效价的兔抗α毒素多克隆抗体作为一抗,HRP和FITC分别标记的羊抗兔IgG作为二抗,通过免疫组化法对α毒素进行了定位检测。结果表明,在小鼠延髓、肠、肾、肺、胃的间充质细胞胞浆中发现了阳性颗粒,在脾、肝的间充质细胞中未发现毒素分布。结果提示,首次在延髓的细胞胞浆中发现了α毒素,表明α毒素能突破血脑屏障进入延髓损坏生命中枢,这可能是引起动物死亡的一个重要原因。In order to test the distribution of alpha-toxin in animals infected by Clostridium perfringens type A,the high titer rabbit anti-alpha-toxin polyclonal antibodies prepared as primary antibodies and HRP or FITC conjugated secondary antibodies were used to detect the alpha-toxin by immunohistochemistry method.Results showed that positive particles were located in the main qualitative cell plasma of medulla,intestine,kidney,lungs and stomach except liver and spleen.The result indicated that the alpha-toxin was firstly found in cell plasma of medulla which broke through the blood brain barrier to damage the vital center.It may be an important reason why the alpha-toxin could cause animal death.

关 键 词：A型产气荚膜梭菌 Α毒素 蛋白表达 免疫组化 

分 类 号：S852.616[农业科学—基础兽医学]