泌乳期山羊腺垂体中ERα和ERβ的分布特点  

Distribution of ERα and ERβ in the Adenohypophysis of the Lactating Goats

作　　者：吕颜枝[1] 杨爽[1] 胡鹏翔[1] 熊东升[1] 耿阳雪[1] 蒋振兴[1] 赵慧英[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨陵712100

出　　处：《动物医学进展》2011年第10期44-46,共3页Progress In Veterinary Medicine

基　　金：西北农林科技大学国家级动物科学实验教学示范中心项目;抗病转基因牛新品种选育重大专项(2009ZX08007-008B)

摘　　要：为了获得ERα和ERβ在泌乳期参与腺垂体激素分泌的调节机理的形态学依据,试验采集泌乳初期、泌乳中期和泌乳后期山羊的腺垂体,用免疫组化SP法检测了ERα和ERβ在腺垂体的分布。结果表明,山羊腺垂体中ER两种亚型分布在腺垂体远侧部的嗜色细胞中;ERα阳性细胞数量随泌乳时间逐渐减少,泌乳初期和泌乳后期多呈胞质阳性,高峰期呈胞核阳性;ERβ的阳性细胞数量在泌乳初期和高峰期较多,泌乳后期较少,多为胞质阳性,高峰期伴有全细胞阳性,泌乳后期伴有个别核阳性。提示不同的泌乳时期ERα和ERβ分别分布在腺垂体远侧部中不同的嗜色细胞,泌乳前期和高峰期主要为PRL细胞,泌乳后乳期主要为嗜碱粒细胞,ERα和ERβ在泌乳各期腺垂体激素分泌中发挥不同的作用。In order to sthdy the distribution characteristics of ERα and ERβ in the adenohypophysis of the lactating goats,the adenohypophysis of in early-lactation,peak lactation and later lactation goats were collected and made to slices to observe the localization of ERα and ERβ positive substance respectively by using immunohistochemical SP method.The results showed the ERα and ERβ positive cells mainly locate in eosinophils in the pars distalis of adenohypophysis.ERα postive cells gradually decrease during lactation period,and mainly locate in cytoplasma in the early lactation and later lactation and in nucleus in peak lactation.The majority of ERβ positive cells scatter in the early lactation and the peak lactation,a few in the later lactation,and the positive products mainly distribute in cytoplasma.Whole-cell are colored in the peak lactation.The result indicated that ERα and ERβ distributed during different chromophil cells in the pars distalis of adenohypophysis In different lactation periods.ERα and ERβ may play a different role in the adenohypophysis hormone secretion during different lactation periods.

关 键 词：山羊腺垂体 泌乳期 雌激素受体 免疫组化 

分 类 号：S852.13[农业科学—基础兽医学]