动物病原高通量检测技术  

Novel Techniques of High-throughput Detection for Animal Pathogenic Microorganisms

作　　者：朱丽萍[1] 颜世敢 

机构地区：[1]山东轻工业学院食品与生物工程学院,山东济南250353 [2]山东省农科院畜牧兽医研究所山东省动物疫病防控与繁育重点实验室,山东济南250100

出　　处：《动物医学进展》2011年第10期103-106,共4页Progress In Veterinary Medicine

基　　金：山东省优秀中青年科学家科研奖励基金(BS2009NY026);山东省农业重大应用技术创新项目资助

摘　　要：当前规模化养殖业迫切需要动物病原检测技术高通量化。高通量检测技术具有高通量、自动化、微量化等特点。病原高通量检测技术可分为高通量免疫学检测(检测抗原)和高通量分子生物学检测(检测核酸)两大类。论文对近年来报道的用于动物病原高通量检测的基因芯片技术、抗体芯片技术、液相芯片技术、流式细胞术多色荧光分析技术、变性高效液相色谱技术等新技术从原理、特点及应用等进行了综述。Presently large-scale breeding urgently requires high-throughput detection techniques for animal pathogenic microorganisms.High-throughput detection techniques have characteristics of high-throughput,automatic,trace,and so on.Animal pathogen high-throughput detection techniques include high-throughput immunological techniques（detection of antigen） and high-throughput molecular biology techniques（detection of nucleic acids）.Recently reported high-throughput detection techniques for animal pathogens were reviewed in the principle,feature and application respects,including gene-chip technology,antibody-chip technology,liquid-chip technology,flow cytometry multi-color fluorescence analysis,denaturing high performance liquid chromatography techniques.

关 键 词：高通量检测 液相芯片 抗体芯片 流式细胞术 基因芯片 变性高效液相色谱技术 病原微生物 

分 类 号：S854.43[农业科学—临床兽医学]