一个水稻多柱头突变体的形态特征和基因定位  

Phenotypic Characterization and Genetic Mapping of an Increased Stigma Mutant in Rice (Oryza sativa L.)

作　　者：周明镜[1,2] 文勇[2] 李双成[1,2] 李成波[2] 张曼华[2] 高烽焱[2] 王玲霞[1,2] 李平[1,2] 

机构地区：[1]四川农业大学水稻研究所,四川温江611130 [2]四川农业大学/作物基因资源与遗传改良教育部重点实验室,四川雅安625014

出　　处：《作物学报》2011年第10期1779-1784,共6页Acta Agronomica Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB100101);国家自然科学基金项目(30800084)资助

摘　　要：水稻产量和品质受花器官发育的直接影响,因此对水稻颖花发育机理的研究有助于水稻产量和品质的遗传改良。在籼稻C2与2480的杂交后代中发现了一个多柱头突变体,与野生型相比,该突变体植株矮化、穗变小、开花延迟和育性降低。颖花解剖发现,其浆片正常,柱头和雌蕊数量增加,雄蕊数目明显减少,并伴随不同程度的雌雄蕊畸形。以突变体作母本,构建群体进行遗传分析,结果显示所有F1均表现正常,F2群体出现3∶1性状分离,证实该突变性状受1对隐性基因控制。利用微卫星标记进行连锁分析,将该基因定位于水稻第6染色体上标记RM3183和RM3827之间,遗传距离分别为2.2cM和12.0cM,且与RM11951、RM19953和RM19961共分离。ISM(t)是一个新的水稻花器官发育控制基因。Floral organ development directly affects rice yield and quality. Here we report a rice floral mutant with increased stigmas, which was isolated from the progenies of the combination of C2/2480. Compared with the wild type rice, the mutant showed dwarf plant, small panicle and grain, curled flag leaf, narrow and opening spikelet, and later flowering. Most of florets were made up of normal lodicule, increased stigmas, more pistils, and fewer stamens. Segregation of wild type plants to mutant plants in three F2 population were accorded with the ratio of 3：1, which indicated that the mutant is controlled by a single recessive gene. With SSR （Simple Sequence Repeat） markers screening in a total of 92 F2 mutant individuals derived from the cross of ism/T116, the mutant gene was located between the molecular markers RM3183 and RM3827, at the distances of 2.2 cM and 12.0 cM, respectively, on chromosome 6, and co-segregated with RM11951, RM19953, and RM19961. ISM（t） is a new floral-organ identity gene located on chromosome 6 in rice. This result provided a foundation of map-based cloning and function analysis of ISM（t） gene.

关 键 词：水稻 花器官 突变体 遗传分析 基因定位 

分 类 号：S511[农业科学—作物学]