产碱性纤维素酶芽孢杆菌ZA-05的分离及功能基因克隆  

Isolation and Functional Gene Cloning of Alkaline Glucanase Producing Bacillus Strain ZA-05

作　　者：魏艳丽[1] 李红梅[1] 李纪顺[1] 扈进冬[1] 杨合同[1] 

机构地区：[1]山东省科学院生物研究所/山东省应用微生物重点实验室,济南250014

出　　处：《湖北农业科学》2011年第18期3859-3863,共5页Hubei Agricultural Sciences

基　　金：国家"863"计划现代农业技术领域重大项目(2006AA10A211)

摘　　要：从造纸污泥中分离到一株芽孢杆菌ZA-05,该菌可产生内切β-1,4-葡聚糖酶酶活性,且在碱性条件下酶活较高,经生理生化和分子生物学鉴定,该菌株为淀粉液化芽孢杆菌(Bacillus amyloliquefaciens);根据GenBank登录的内切-β-1,4-葡聚糖酶基因(DQ782954.1,M28332.1,AY859492.1)的同源性序列,利用PCR方法克隆到该菌株的功能基因,并对其进行测序。测序结果显示其全长为1 500 bp,推测其含499个氨基酸。An alkaline glucanase producing strain ZA-05 which had the enzyme activity of endo-β-1,4-glucanase was isolated from paper mill sludge by Congo-red staining method.It was identified as Bacillus amyloliquefaciens based on its morphological characters and 16S rDNA sequence.According to the homologous sequence of endo-β-1,4-glucanase gene（GenBank No.DQ782954.1,M28332.1,AY859492.1）,an alkali-tolerant glucanase gene was amplified by PCR and then sequenced.The full length of this alkali-tolerant glucanase gene was 1 500 bp and presumably encoded 499 amino acids.

关 键 词：淀粉液化芽孢杆菌 分离鉴定 碱性纤维素酶 基因克隆 

分 类 号：Q939.124[生物学—微生物学]