海洛因成瘾大鼠脑一氧化氮、丙二醛、血清超氧化物歧化酶的变化  

THE CHANGES OF NO,MDA AND SOD IN BRAINS OF HEROIN-ADDICTED RATS

作　　者：韦献良[1] 李百玮[1] 

机构地区：[1]广西医科大学人体解剖学教研室,南宁530021

出　　处：《广西医科大学学报》2011年第4期496-498,共3页Journal of Guangxi Medical University

基　　金：广西自然科学基金资助项目(No.桂科自0832128);广西教育厅科研项目(No.201010LX053)

摘　　要：目的:通过动物实验观察海洛因成瘾大鼠脑组织一氧化氮(NO)、丙二醛(MDA)和血清超氧化物歧化酶(SOD)的变化,探讨海洛因成瘾致脑损伤的病理机制。方法:将20只成年雌性SD大鼠随机分为海洛因模型组和生理盐水对照组。行纳洛酮激发试验,按照Maldonado戒断症状评分标准判断海洛因成瘾强度。采用化学比色法检测大鼠脑额叶皮质、海马、间脑、小脑和脑干5个脑区的NO、MDA和SOD含量。结果:与生理盐水对照组比较,海洛因成瘾模型组大鼠纳洛酮激发试验阳性;额叶皮质、海马、间脑、小脑和脑干的NO、MDA含量明显升高(P<0.05,P<0.01),SOD含量明显降低(P<0.05,P<0.01)。上述各脑区NO与MDA的含量变化呈正相关(r=0.175,P<0.05);NO与SOD的含量变化呈负相关(r=-0.384,P<0.01);MDA与SOD的含量变化呈负相关(r=-0.358,P<0.05)。结论:海洛因成瘾脑组织出现自由基氧化损伤,引发脑组织一系列结构及功能变化,这可能是海洛因成瘾脑损害的一个病理机制。Objective： To explore the mechanism of heroin addiction-induced brain injury by observing the changes of nitric oxide （NO）, malondialdehyde （MDA）, superoxide dismutase （sOD） in animal experiments. Methods： Twenty adult female Sprague-Dawley （SD） rats were divided into two groups： heroin model and control groups. After the establishment of animal model, the rats were given the intraperitoneal injection of naloxone, heroin addiction strength was judged by the Maldonado addiction withdrawal symptoms score criteria. Ten rats in each group were decapitated the brain and the brain were separated into frontal cortex, hipocampus, diencephalon, cerebellum and brain stem, then the changes of NO, MDA, and SOD were measured by chemical colorimetric method in each brain parts. Results：Compared with those rats in the control group,the naloxone test of heroin addiction model rats was positive. The contents of NO and MDA in the frontal cortex, hipocampus, diencephalon, cerebellum and brain stem of rats in model group was significantly increased （ P 〈0.05 and P 〈0.01）. The contents of SOD of rats in model group were significantly decreased in the same brain area（ P〈0.05 and P 〈0. 01）. In brains of heroin addiction model rats,the contents of NO were positively correlated with MDA （ P〈0.05）, the contents of NO were negatively correlated with SOD （ P 〈0.05） ; the contents of MDA were negatively correlated with SOD （ P 〈0.05）. Conclusion：The oxidative injury of free radical in the brain tissues of heroin addicted rats leads to a series of changes of brain structure and function, which may be a pathological mechanism of the heroin addiction-induced brain injury.

关 键 词：海洛因成瘾 脑 NO MDA SOD 

分 类 号：R996[医药卫生—毒理学]