叉状头/翅膀状螺旋转录因子在BXSB狼疮小鼠肾脏中的表达  

Expression of Forkhead/winged Helix Transcription Factor in Renal Tissues of BXSB Mice

作　　者：王玮炜[1] 黄安斌[1] 杜戎[1] 刘宇宏[1] 宋优[1] 沈凌汛[1] 余立凯[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院风湿免疫科,武汉430022

出　　处：《华中科技大学学报（医学版）》2011年第5期567-570,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的观察叉状头/翅膀状螺旋转录因子(Foxp3)在BXSB狼疮小鼠肾脏中的表达,探讨Foxp3在狼疮性肾炎中可能的免疫耐受诱导作用及机制。方法选取8周龄及16周龄雄性BXSB小鼠各6只,以8周龄C57BL/6雄性小鼠6只为正常对照,用免疫组织化学及实时荧光定量PCR方法,检测Foxp3在小鼠肾脏中的表达。结果正常对照组C57BL/6小鼠肾组织Foxp3阳性表达,细胞呈深棕色着色,广泛分布于肾小管;8周龄及16周龄BXSB小鼠肾脏组织Foxp3弱阳性表达,细胞呈浅棕色着色,分布于肾小管。8、16周龄BXSB组小鼠肾组织内Foxp3mRNA表达水平[(0.55±0.06),(0.51±0.07)]均较正常对照组(1.02±0.08)降低,差异有统计学意义(均P<0.01);16周龄BXSB组小鼠的Foxp3mRNA表达水平较8周龄BXSB组降低,但差异没有统计学意义(P>0.05)。结论 BXSB小鼠狼疮肾炎的发病机制可能与调节性T细胞的Foxp3表达下调相关。Objective To investigate the expression of forkhead/winged helix transcription factor（Foxp3）in the kidney of BXSB mice and its potential role in inducing the immune tolerance pathogenesis of lupus nephritis in BXSB mice. MethodsFoxp3 was detected in controls,8-week BXSB male mice and 16-week BXSB male mice by using immunohistochemistry and real-time PCR. Results Foxp3 protein staining in control group was strong as dark brown and distributed in renal tubules,but in 8-and 16-week BXSB mice the staining was weaker as light brown than controls.The mRNA expression of Foxp3 was significantly decreased in renal tissues of 8-and 16-week BXSB mice as compared with that in the controls[（0.55±0.06） and（0.51±0.07） vs（1.02±0.08）,P0.01],but there was no significant difference between 8-and 16-week BXSB mice（P0.05）. Conclusion Down-regulation of Foxp3 in regulatory T cells may be involved in the pathogenesis of lupus nephritis in BXSB mice.

关 键 词：叉状头/翅膀状螺旋转录因子 狼疮肾炎 调节性T细胞 BXSB小鼠 

分 类 号：R593.241[医药卫生—内科学]