检测H5亚型禽流感的压电免疫传感器的研究  

Study on Piezoelectric Immunosensor for the Detection of H5 Sera-subtype Avian Influenza

作　　者：刘靖清 卞红春 胡云发 王新卫[3] 陈枝楠 陈勇 詹爱军 

机构地区：[1]盐城市农业委员会,江苏盐城224000 [2]深圳市检验检疫科学研究院,广东深圳518010 [3]河南农业大学牧医工程学院,河南郑州450002

出　　处：《现代生物医学进展》2011年第20期3838-3840,3856,共4页Progress in Modern Biomedicine

基　　金："十一五"国家科技支撑项目(2006BAK20A29);广东省科技重大专项计划项目(2004A2090102)资助

摘　　要：目的:为研制检测H5亚型禽流感的压电免疫传感器。方法:用巯基丙酸在镀银电极石英晶体自组装巯基丙酸单分子膜再通过N-乙基-N′(-3-二甲氨基)丙基碳化二亚胺盐酸(EDC)和N-羟基琥珀酰亚胺(NHS)偶联抗H5亚型禽流感病毒的特异性单抗构建传感器芯片,建立了可以检测H5亚型禽流感病毒的免疫传感器。结果:结果表明,该法具有较好的特异性,不与H9亚型流感病毒和NDV反应;检测灵敏度达到10-50个EID50。结论:本文结果为检测禽流感病毒免疫传感器的进一步深入研究奠定了基础,这为其它相关病毒的监测提供了一种新途径。Objective： The aim is to develop the piezoelectric immunosensor to detect H5 avian influenza （AI）. Methods： Set up the immunosensor by self-assembling Mercaptopmpionic acid（MPA） to be monolayer on the silver-coated electrode of quartz crystal and coupling the monoclonal antibody to H5 AIV with N-ethy-N＇ -（3-dimethyl aminopropyl）carbodiimide hydrochloride（EDC） and N-hydr oxysuccinimide （ NHS）,the chip of immunosensor to detect H5 AIV was established. Results： The results showed that the immunosensor displayed better specificity to H5 AIV and no respond to H9AIV and NDV when being used for detection. The sensitivity test indicated the detecting limit for the H5 AIV could reach to 10-50 EID50. Conclusion： The method provided a foundation for further research on immunosensor for detecting AIV and it could be a new exploration to detect other viruses with the same technique in animal husbandry.

关 键 词：压电免疫传感器 生物自组装法 H5亚型禽流感病毒 

分 类 号：S855.3[农业科学—临床兽医学]