Penicillium chrysogenum的低温空气等离子体诱变研究  

Studies of air plasma techniques in mutating Penicillium chrysogenum

作　　者：桂芳[1] 刘会[1] 王辉[1] 王鹏[1] 袁成凌[1] 郑之明[1] 

机构地区：[1]中国科学院离子束生物工程学重点实验室,合肥230031

出　　处：《辐射研究与辐射工艺学报》2011年第5期290-296,共7页Journal of Radiation Research and Radiation Processing

基　　金：国家高技术研究发展计划(2009AA02Z305)资助

摘　　要：以产黄青霉菌(Penicillium chrysogenum)Pc05为出发菌株,利用物理诱变方法低温空气等离子体技术对其进行诱变处理,结果表明:在处理时间为30 min内时,产黄青霉孢子的存活率随处理时间变化呈现出明显的"马鞍型"曲线;正突变株占所有突变株比例为44.19%,且负突变率很低。经过初筛、复筛获得突变株aPc051310,最终其青霉素效价比出发菌提高了42.1%。化学活性粒子和带电粒子的协同作用被认为是诱变发生的原因。实验结果证明低温空气等离子体技术作为一种新的工业微生物菌种诱变方法,具有应用潜力。Penicillin producing strain Penicilliurn chrysogenum Pc05 as the starting strain was mutated by low-temperature air plasma technology. As the result revealed, in 30 minutes, the survival rate of spores followed the saddle-shaped curve. The positive mutants accounted for 44.19% of all mutants while the negative mutation was low. After primary and secondary screening, the mutant aPc051310 was obtained, and eventually its penicillin titer increased 42.1% compared with that of starting strain. Synergetic effect between chemical reactive species and charged particles was considered as the main mutation mechanism involved in low temperature air plasma. All the results have been proved that as a new industrial microbial strains mutation method, low temperature air plasma has potential applications.

关 键 词：低温空气等离子体 产黄青霉 诱变育种 青霉素 诱变机理 

分 类 号：Q689[生物学—生物物理学] Q939.97