通心络下调基质金属蛋白酶稳定动脉粥样硬化斑块的作用研究  

A study on Tongxinluo down-regulating matrix metal proteinase-2/9 levels to stabilize atherosclerotic plaque

作　　者：陈永斌[1] 蔡志玲[1] 冷静[2] 樊继波[3] 

机构地区：[1]广西医科大学第一附属医院,广西南宁530021 [2]广西中医学院,广西南宁530001 [3]三峡大学第一临床医学院,湖北宜昌443101

出　　处：《中国中西医结合急救杂志》2011年第5期273-276,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：广西自然科学基金资助项目（桂科自0832150）

摘　　要：目的通过观察通心络调节基质金属蛋白酶-2／9（MMP-2／9）的活性及稳定动脉粥样硬化（As）斑块的效应，探讨通心络治疗急性冠脉综合征（ACs）的有效机制。方法应用球囊损伤腹主动脉加高脂饮食建立兔As模型。将38只新西兰兔按数据列表法分为手术对照组（A组，6只）、As模型组（B组，8只）、通心络治疗组（C组，8只）、辛伐他汀治疗组（D组，8只）和通心络＋辛伐他汀治疗组（E组，8只）。给药8周后检测血脂；用多普勒检测腹主动脉血流速度；用免疫组化染色检测MMP-2／9的表达。结果B组MMP-2／9表达、血脂水平较A组明显升高；与B组比较，C、D、E组MMP-2／9阳性表达（A值）明显减少，以E组表达水平最低（MMP-2：25651．7±2185．8比846907．8±32285．6，MMP-9：24258．1±1499．5比942219．5±21857．9，均P〈0．05）；C、D、E组血清总胆固醇（TC，mmol／L）、三酰甘油（TG，mmol／L）、低密度脂蛋白（LDL，mmol／L）均有不同程度降低，以E组降低最显著（TC：32．51±0．88比38．56±1．91，TG：7．15±1．87比8．07±1．68，LDL：25．47±3．56比32．58±2．14，均P〈0．05）；B、C、D、E组较A组腹主动脉血流速度下降，以E组下降趋势最缓慢（m／s：21．55±0．78比35．95±1．34，P〈0．05）。结论通心络有加快血流速度及减少AS斑块形成的作用，其机制可能与下调MMP-2／9及其相关因子表达、稳定斑块有关。Objective Through observing the effect of Tongxinluo on regulating matrix metal proteinase （MMP-2/9） levels and stabilizing the atherosclerosis （AS） plaques to discuss the effective mechanism of Tongxinluo for treatment of acute coronary syndrome （ACS）. Methods Thirty-eight New Zealand rabbits were randomly divided into five groups., normal （A group, 6 cases）, model （B group, 8 cases）, Tongxinluo treated （C group, 8 cases）, simvastatin treated （D group, 8 cases） and Tongxinluo plus simvastatin treated （E group, 8 cases） groups. A ball pouch was applied to damage abdomen aorta and a high fat diet was used to establish rabbit AS model. The rabbits in medicine treated groups were given respective medicine and the blood lipid was detected 8 weeks later） simultaneously the blood stream velocities of iliac artery and aorta were examined with Doppler apparatus and MMP-2/9 levels were detected through immunohistochemistry. Results MMP-2/9 expressions and blood lipid level in B group were higher than those in A group） compared with B group, MMP-2/9 positive expressions were significantly lower in C, D and E groups, being the lowest in E group （MMP-2.. 25 651.7±2 185.8 vs. 846 907.8±32 285.6, MMP-9： 24 258.1±1 499.5 vs. 942 219.5± 21 857.9, all P〈0.05）） total cholesterol （TC, mmol/L）, triglyceride （TG, mmol/L） and low density lipoprotein （LDL, mmol/L） in serum all were decreased in certain degrees of level in C, D and E groups, being reduced the most in E group （TC.. 32. 51±0. 88 vs. 38.56±1.91, TG： 7. 15±1.87 vs. 8. 07±1.68, LDL： 25. 47±3.56 vs. 32. 58±2.14, all P〈0. 05）; compared to A group, the velocities of the blood stream were all decreased in B, C, D and E groups, being reduced the most in E group （m/s： 21.55±0.78 vs. 35.95±1.34, P〈0.05）. Conclusion Tongxinluo can speed up the blood flow velocity and reduce the AS plaque formation, which may be related to the stabilization of plaques through decreasing MMP-2/9 and associated fact

关 键 词：通心络 动脉粥样硬化 急性冠脉综合征 基质金属蛋白酶 

分 类 号：R285.5[医药卫生—中药学] R541.4[医药卫生—中医学]