石家庄市水环境中嗜肺军团菌AFLP分型研究  

AFLP Typing of Legionella pneumophila in Water Environment in Shijiazhuang

作　　者：秦丽云[1] 张鑫 郭玉梅[1] 徐保红[1] 陈慧巧[3] 王苋[1] 吕国平[1] 曹春红[1] 

机构地区：[1]石家庄市疾病预防控制中心微生物检验所,河北050011 [2]石家庄市桥西区疾病预防控制中心 [3]石家庄市第二医院检验科

出　　处：《环境与健康杂志》2011年第10期908-910,共3页Journal of Environment and Health

摘　　要：目的对石家庄市水环境中分离到的嗜肺军团菌进行分子分型，研究嗜肺军团菌AFLP分型特征，为建立嗜肺军团菌分子型别库提供资料。方法运用欧洲军团菌感染工作组（European Working GrouponLegionella Infection，EWGLI）组织制定的扩增片断长度多态性分型（amplifiedfragmentlengthpolymorphism，AFLP）方法，对石家庄市水环境中分离到的39株嗜肺军团菌进行分子分型，对分型结果进行聚类分析，探讨菌株间的相互关系。结果按照血清分型方法，将水样中嗜肺军团菌分为Lp1型和，Jp2～14型。运用AFIJP技术对39株嗜肺军团菌菌株分型，其中，将31株Lp1血清型分为18种AFLP型，分别记为AFLP1～AFLP18，以AFLP1型与AFLP11型为主要型别；8株幻2～14血清型分为7种AFLP型，分别记为AFLPI～AFLPVII，以AFLPm为主要型别；在同一地区嗜肺军团菌菌株问，相同血清型别的相似性系数存在差异较大，但仍存在优势株；不同地区之间有部分菌株相似性系数为100％，表明不同地区的水样嗜肺军团菌菌株来源同一性。结论AFLP分型所得到的不同图谱表明各嗜肺军团菌株基因型呈多态性。Objective To study the molecular genotyping of Legionella pneumophila strains isolated from different water environment in Shijiazhuang and dominant species and species distribution. Methods Amplified fagment length polymorphism （AFLP） made by the European Working Group for LegioneUa Infections was used for typing Legionella from water environment in Shijiazhuang. Cluster analysis was used to explore the relationships among the strains. Results According to the serum typing methods,Legionella in water were classified into Lp 1 ,Lp2-14. The use of AFLP technology for 39 strains of Legionella and serogroup 14）1 were classified into 18 types,denoted as AFLP1-AFLP18,AFLPI type and AFLP11 type as the main type; The 9 strains of serotypes Lp2-14 were classified into 7 types,denoted as AFLP 1 -AFLP VII ,AFLP m as the main type. In the same area of LegioneUa pneumophila strains,the same serum type similarity coefficient had difference, but there was still the dominant strain; between different regions of some strains of similarity coefficient of 100%, showed that the Legionella strains belonged to the same source of different regions of the water. Conclusion Every genotype isolate presents polymorphic, which can control typing information of LegioneUa pneumophila molecular.

关 键 词：嗜肺军团菌 AFLP分型 血清分型 

分 类 号：R123.1[医药卫生—环境卫生学]