水中α-萘胺的重氮偶合分光光度测定法  

Determination of α-Naphthylamine Concentration in Water Based on Diazotion-Coupling Reaction by Spectrophotometry

作　　者：史云峰[1,2] 赵牧秋[1,2] 张丽莉[2] 

机构地区：[1]琼州学院生物科学与技术学院,海南三亚572022 [2]中国科学院沈阳应用生态研究所

出　　处：《环境与健康杂志》2011年第10期911-913,共3页Journal of Environment and Health

基　　金：国家科技支撑计划项目(2011BAD11B04);琼州学院青年教师科研基金项目(QYQN201123)

摘　　要：目的建立水中α-萘胺浓度的6种重氮偶合反应体系分光光度测定法。方法在pH=2.2的酸性条件下,向含有α-萘胺的水样中分别加入0.01 mol/L苯胺、对氨基苯磺酸、对氨基苯磺酰胺、对氨基苯甲酸、对氨基苯乙酮或对硝基苯胺溶液,重氮盐偶合显色反应15 min后,采用分光光度法分别在540、530、520、520、520和475 nm处进行测定。结果 6种显色体系的线性范围分别为0.031～16.0、0.016～14.0、0.014～12.5、0.022～15.5、0.016～13.5和0.046～20.0 mg/L,所得α-萘胺的回归方程均呈较好的线性关系,检出限分别为0.031、0.016、0.014、0.022、0.016和0.046 mg/L;平均回收率为95.8%～105.6%,RSD为1.74%～4.58%。同时采用该6种方法与气相色谱法测定废水α-萘胺浓度,差异均无统计学意义。结论 6种方法均简单、快捷、准确度高,可用于水中α-萘胺浓度的快速测定。Objective To establish the spectrophotometric methods for determining α-naphthylamine concentration in water based on diazotion-coupling reaction. Methods α-naphthylamine coupled with aniline ,4-aminobenzene sulfonic acid,sulfanilic amide,p-aminobenzoie acid,4-aminoacetophenone or p-nitroaniline in sodium nitrite medium to form colored dye under the acidic condition of pH=2.2. The maximum absorption wavelengths were 540,530,520,520,520 and 475 nm respectively. Results The linear range were 0.031-16.0,0.016-14.0,0.014-12.5,0.022-15.5,0.016-13.5 and 0.046-20.0 mg/L respectively,and the limit of detection were 0.031,0.016,0.014,0.022,0.016 or 0.046 rag/L, respectively. The rates of recovery were 95.8%-105.6% and RSDs were 1.74%-4.58%,respectively. Compared with gas chromatography ,no significant difference presented using the methods to determine α-naphthylamine concentration in waste water. Conclusion The methods are simple, accurate and applicable to the determination of α-naphthylamine concentration in water.

关 键 词：Α-萘胺 重氮偶合 分光光度法 

分 类 号：O657.31[理学—分析化学]