大孔吸附树脂分离纯化单叶铁线莲总皂苷的研究  

Separation and purification of total saponins from Clematis henryi by macroporous resins

作　　者：孙晓博[1,2] 张凯[1,3] 李兰林[1,3] 向大雄[1,3] 

机构地区：[1]中南大学湘雅二医院湖南省中药制剂新技术重点研究室,湖南长沙410011 [2]益阳市中心医院,湖南益阳413000 [3]中南大学药学院,湖南长沙410013

出　　处：《中草药》2011年第10期1977-1981,共5页Chinese Traditional and Herbal Drugs

基　　金：湖南省中医药科研计划项目(2010042)

摘　　要：目的研究大孔吸附树脂分离纯化单叶铁线莲总皂苷的工艺条件,为单叶铁线莲总皂苷的工业化生产提供实验依据。方法采用比色法测定总皂苷;比较了5种大孔吸附树脂的静态饱和吸附量、静态解吸率,筛选出适宜的树脂;对最佳树脂进行动态吸附、解吸试验,确定最佳的吸附洗脱工艺。结果 D-101大孔树脂对单叶铁线莲总皂苷有较好的吸附分离性能。最佳工艺条件为:上样液质量浓度为生药0.2 g/mL,pH值为5,上样体积流量为1 BV/h;4 BV蒸馏水洗脱杂质;4 BV 70%乙醇洗脱总皂苷,洗脱体积流量2 BV/h。按此工艺条件生产的总皂苷质量分数达到71.53%,收率86.02%,精制度达238.81%。结论 D-101大孔吸附树脂适合富集纯化单叶铁线莲中总皂苷。Objective To study the technological parameters of enriching and purifying total saponins from Clematis henryi by macroporous resins.Methods The content of saponins was determined by colorimetry;The optimum macroporous resins were selected by comparing static adsorptive capabilities and desorption ratio of five kinds of resins;The dynamic adsorption and desorption of D-101 resin were researched to obtain the optimum parameters.Results The D-101 resin showed the best adsorptive and desorptive capabilities;The optimum technological parameters were obtained as follows：The concentration of sample solution is crude drug 0.2 g/mL,the pH value of sample solution is 5 at flow rate of 1 BV/h;Then washed with 4 BV purified water,the desorption of saponins was washed with 70% ethanol at washing rate of 2 BV/h.The purity of total saponins obtained was 71.53%,the transfer ratio reached 86.02% and refine ratio reached 238.81%.Conclusion Using D-101 resin to enrich and purify total saponins from C.henryi is feasible.

关 键 词：单叶铁线莲 总皂苷 大孔吸附树脂 分离纯化 比色法 

分 类 号：R284.2[医药卫生—中药学]