虎杖苷对脓毒症致急性肾损伤小鼠的保护作用  

Protection of polydatin on sepsis-induced acute kidney injury in mice

作　　者：吴孟娇[1] 李晓会[1] 郑佳佳[1] 张丽娜[1] 张力[2] 万敬员[1] 

机构地区：[1]重庆医科大学重庆市生物化学与分子药理学重点实验室,重庆400016 [2]重庆医科大学病理生理教研室,重庆400016

出　　处：《中草药》2011年第10期2033-2036,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81072650)

摘　　要：目的观察虎杖苷对盲肠结扎穿孔(cecal ligation and puncture,CLP)所致脓毒症诱导的急性肾损伤小鼠的影响,并初步探讨其作用机制。方法建立小鼠CLP诱导的脓毒症致急性肾损伤模型,随后用不同剂量的虎杖苷(50、100、300 mg/kg)干预,观察小鼠一般情况,并于手术24 h后,用试剂盒方法测定血清尿素氮(BUN)、肌酐(Cr)水平,ELISA法测定血清白细胞介素-6(IL-6)表达量,Western blotting法检测肾脏诱导型一氧化氮合酶(iNOS)蛋白水平,并取肾脏组织进行病理分析。结果与模型组相比,虎杖苷明显改善小鼠一般情况,同时降低血清BUN、Cr、IL-6及肾脏中iNOS蛋白水平,缓解肾脏组织损伤,并呈一定的剂量依赖关系。结论虎杖苷对脓毒症致急性肾损伤具有保护作用,其作用机制可能与其抑制血清IL-6及肾脏中iNOS蛋白表达有关。Objective To investigate the protective effect and mechanism of polydatin（PD） on sepsis-induced acute kidney injury in mice suffered from cecal ligation and puncture（CLP）.Methods The mice were divided into five groups：model group,Sham group,and PD（50,100,and 300 mg/kg） groups.The model of septic kidney injury in Kunming mice was made by CLP.One hour before the operation,PD and saline were ip administered into mice,respectively.Twenty-four hours after the operation,mice were decapitated for the measurement of urea nitrogen（BUN）,serum creatinine（Cr） levels in serum using kit,and interleukin-6（IL-6） and inducible nitric oxide synthase（iNOS） were detected by ELISA and Western blotting,respectively.Besides,the kidney tissue was assessed pathologically and kidney injury was observed histologically.Results Compared with CLP group,the levels of BUN,Cr,IL-6,and iNOS were much lowered in PD groups.In addition,PD significantly attenuated the pathologic changes in kidney tissues induced by sepsis in a dose-dependent manner.Conclusion PD has a protective effect against sepsis-induced acute kidney injury.The mechanism may be related to the inhibition on the expression of IL-6 in serum and iNOS protein in kidney tissues.

关 键 词：虎杖苷 脓毒症 肾损伤 盲肠结扎穿孔(CLP) 白细胞介素-6 诱导型一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]