斑马鱼模型在中药活性筛选中的适用性研究  

Adaptability of zebrafish model in activity screening of Chinese materia medica

作　　者：韩利文[1,2] 袁延强[2] 何秋霞[2] 王思锋[2] 李遇伯[1] 楚杰[2] 刘可春[2] 刘昌孝[3] 

机构地区：[1]天津中医药大学中药学院,天津300193 [2]山东省科学院生物研究所生物传感器重点实验室。山东济南250014 [3]天津药物研究院药代动力学和药效动力学省部共建国家重点实验室,天津300193

出　　处：《中草药》2011年第10期2037-2041,共5页Chinese Traditional and Herbal Drugs

基　　金：山东省自然科学基金资助项目(SY2008C179);山东省科技发展计划项目(2006GG3202038)

摘　　要：目的考察斑马鱼血管生成抑制模型在中药各类样品活性筛选中的适用性。方法选用TG(VEGFR2:GFP)系血管荧光转基因斑马鱼和AB系斑马鱼作为筛选模型,分别用中药单体靛玉红、单味药材(虎杖、长春花)提取物、复方(西黄丸)处理斑马鱼胚胎,以节间血管数作为指标,考察不同类型中药样品对斑马鱼胚胎血管生成的影响。结果靛玉红高剂量(50μg/mL)明显抑制斑马鱼节间血管的生成(P<0.05);虎杖醋酸乙酯提取部位随着质量浓度的升高抑制作用增强,10μg/mL时与对照组比较差异显著(P<0.05);长春花醋酸乙酯提取部位100μg/mL时明显抑制斑马鱼血管生成(P<0.01),同时未见胚胎死亡;西黄丸质量浓度大于50μg/mL时,抑制斑马鱼血管生成作用与对照组比较差异显著(P<0.05),100μg/mL时呈非常显著的统计学差异(P<0.01)。结论斑马鱼模型对于成分复杂的中药样品具有很好的适应性,这对于从我国丰富的中药资源中发现结构新颖、活性独特的先导化合物具有重要价值。Objective To investigate the adaptability of zebrafish anti-angiogenesis model in activity screening of Chinese materia medica.Methods Two strains of zebrafish,TG（VEGFR2：GFP） transgenic zebrafish and AB zebrafish,were used for the screening models.Intersegmental vessel number was considered as an index for angiogenesis inhibition when different types of samples such as indirubin,extracts of Polygoni Cuspidati Rhizoma et Radix and Catharanthi Herba,and Compound Xihuang Pills were administrated to zebrafish embryo.Results High-dose group of indirubin（50 μg/mL） could obviously inhibit intersegmental vessel of zebrafish（P0.05）.The EtOAc extract of Polygoni Cuspidati Rhizoma et Radix showed higher inhibitory activity with the concentration increased and there was a significant difference at 10 μg/mL compared with control group（P0.05）.The EtOAc extract of Catharanthi Herba at 100 μg/mL significantly inhibited angiogenesis of zebrafish embyro（P0.01） and no embyro death was found.Significant difference was found when the concentration of Compound Xihuang Pills was above 50 μg/mL when compared with control group and 100 μg/mL group showed obvious significance（P0.01）.Conclusion Zebrafish model shows good adaptability for Chinese materia medica samples with complex constituents,which is very valuable for discovering the leading compound with novel structure and unique activity from abundant traditional herb resources in China.

关 键 词：斑马鱼 血管生成 中药 药物筛选 先导化合物 

分 类 号：R285.5[医药卫生—中药学]