冬凌草甲素注射剂诱导人胃癌SGC-7901细胞凋亡及其机制研究  

Inducement of Oridonin Injection to SGC-7901 cell apoptosis and its mechanisms

作　　者：季宇彬[1,2] 冮剑[1,2] 高世勇[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076

出　　处：《中草药》2011年第10期2051-2055,共5页Chinese Traditional and Herbal Drugs

基　　金：黑龙江省新世纪优秀人才支持计划(1251-NCET-019);黑龙江省研究生创新基金项目(YJSCX2011-163HLJ)

摘　　要：目的研究冬凌草甲素注射剂诱导人胃癌SGC-7901细胞凋亡及与细胞内Ca2+浓度([Ca2+]i)的关系。方法 MTT法检测冬凌草甲素注射剂对SGC-7901细胞的细胞毒活性;倒置显微镜观察冬凌草甲素注射剂对细胞形态学的影响;流式细胞术测定细胞凋亡率;激光共聚焦显微术检测SGC-7901细胞[Ca2+]i的变化;JC-1单染,激光共聚焦显微术检测SGC-7901细胞中线粒体膜电位的变化。结果冬凌草甲素注射剂对SGC-7901细胞的IC50为21.74μmol/L;作用48 h后,细胞生长密度变疏,细胞皱缩,其中冬凌草甲素注射剂高浓度组大部分细胞破碎。冬凌草甲素注射剂5.5、11、22μmol/L作用24 h后SGC-7901细胞凋亡率分别为7.07%、18.57%、22.96%;钙离子荧光强度高于对照组;线粒体膜电位荧光强度显著低于对照组。结论冬凌草甲素注射剂通过升高SGC-7901细胞[Ca2+]i诱导细胞凋亡。Objective To study the apoptosis of Oridonin Injection against human gastric carcinoma SGC-7901 cell line and its relationship with intracellular calcium concentration（[Ca2＋]i）.Methods The cytotoxicy of Oridonin Injection against SGC-7901 cells was evaluated by MTT assay.Morphology of SGC-7901 cells was observed by inverted microscope.Cell apoptosis rate was measured by flow cytometry.[Ca2＋]i of SGC-7901 cells and mitochondrial membrane potential（MMP）were determined by laser scanning confocal microscope.Results IC50 of Oridonin Injection to SGC-7901 cells was 21.74 μmol/L.Cell density decrease,cell shrinkage,and cell breakage in high-dose group were observed 48 h after administration.The apoptosis rate values of 5.5,11,and 22 μmol/L Oridomin Injection groups 24 h after administration were 7.07%,18.57%,and 22.96%,respectively.Fluorescence intensity（FI） values of [Ca2＋]i were significantly higher than those in control group.And FI values of MMP were significantly lower than those in control group.Conclusion Oridonin Injection could induce cell apoptosis by increasing [Ca2＋]i of SGC-7901 cells.

关 键 词：冬凌草甲素注射剂 人胃癌SGC-7901细胞 细胞凋亡 钙离子 线粒体膜电位 

分 类 号：R282.71[医药卫生—中药学] R979.19[医药卫生—中医学]