特效植物营养素对水稻基因表达的表观遗传效应初探  

Primary exploration on the epigenetic effects of SPNE on gene expression of rice

作　　者：谭锬[1] 梁前进[1] 余素芹[2] 王正询[2] 谢国文[2] 江奕君[3] 高云[3] 

机构地区：[1]北京师范大学生命科学学院/基因工程药物及生物技术北京市重点实验室,北京100875 [2]广州大学生命科学学院,广东广州510006 [3]广东省农科院水稻研究所,广东广州510640

出　　处：《广东农业科学》2011年第20期12-15,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(30971470);广东省自然科学基金(8151009101000027);广州市属高校科技计划项目(08C038);北京师范大学抗性基因资源与分子发育北京市重点实验室开放基金(2011-02)

摘　　要：用特效植物营养素(Special Plant Nutritional Element,简称SPNE)处理不同水稻品种,比较处理组和对照组水稻的F1代幼苗及不同代系处理组和对照组的水稻幼苗的生长及过氧化物同工酶和基因转录的差异。结果表明,SPNE对F1代水稻幼苗的生长表型及表观遗传学表型影响较大,其中对上代用SPNE处理过的穗珍香常规稻的幼苗(F1)株高和过氧化物同工酶酶谱有显著影响,对cDNA的RAPD分析有一定影响。此外,对两代连续用SPNE处理的水稻的分析表明,同工酶和基因转录差异可部分传代。说明SPNE对水稻过氧化物同工酶和基因转录有明显影响,并具有表观遗传特征。Treating different varieties of rice by using SPNE. Comparing the growth and the differences of peroxidase isozyme and gene transcription （by PAPD of eDNA） in FI seedlings between the treated group and the control and between the multi-generation treated group and the control group. Results showed that SPNE distinctly affected the growth and epigenetie phenotypes of Ft rice seedlings. The effect on the plant height of FI from the system that their parents had been treated with SPNE was distinct, so was the effect on peroxidase isozyme of regular rice variety Suizhenxiang. The result of eDNA RAPD also showed a certain differences. In addition, it was suggested by analyzing the rice with treating for two successive generations that the changes in isozyme and gene transcription may partly hereditable. SPNE may distinctly affect peroxidase isozyme and gene transcription of rice, and these effects have epigenetic characteristics.

关 键 词：特效植物营养素 表观遗传 同工酶 RAPD 

分 类 号：Q319.2[生物学—遗传学]