Comparison on Four Extraction Methods of Genomic DNA from Clematis fasciculiflora Franch  

4种滑叶铁线莲基因组DNA提取方法比较(英文)

作　　者：胡祎晨[1] 孙正海[1] 王锦[1] 李世峰[2] 辛培尧[1] 范萱[1] 

机构地区：[1]西南林业大学园林学院,云南昆明650224 [2]云南省农业科学院花卉研究所,云南昆明650205

出　　处：《Agricultural Science & Technology》2011年第10期1420-1423,共4页农业科学与技术（英文版）

基　　金：Supported by Applied Basic Research Project of Yunnan Province(2010ZC089);the948Project of National Forestry Bureau(2008-4-11);Sharing Platform Project of Provincial and Ministerial Key Subject,Key Laboratory and School Laboratory of Provincial Colleges and Universities in Yunnan Province;Science and Technology Innovation Fund of Southwest Forestry University~~

摘　　要：[Objective] This study aimed at comparing the four extraction methods of genomic DNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch.[Method] Leavies of Clematis fasciculiflora Franch were used as materials for comparing the purity and concentration of extracted DNA and extracting time among the four extraction methods of genomic DNA including improved CTAB method Ⅰ,improved CTAB method Ⅱ,improved CTAB method Ⅲ and improved SDS method.[Result] The four extraction methods could all be successfully used for extracting the genomic DNA from Clematis fasciculiflora Franch.The purity of genomic DNA was the highest using improved CTAB method Ⅰ,with the longest extracting time;while the concentration of genomic DNA was the maximum using the improved SDS method,with the shortest extracting time and relatively low purity;the extracting time of improved CTAB method Ⅲ was the shortest.[Conclusion] This study had established the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch and supported for the further research using molecular biological methods.[目的]对4种滑叶铁线莲基因组DNA提取方法进行比较研究,建立滑叶铁线莲最适的DNA提取方法。[方法]以滑叶铁线莲叶片为材料,比较改良CTAB法Ⅰ、改良CTAB法Ⅱ、改良CTAB法Ⅲ、改良SDS法这4种基因组DNA提取法在提取的DNA纯度、浓度和提取时间等方面的不同。[结果]4种方法都可提取滑叶铁线莲基因组DNA。改良CTAB法Ⅰ提取DNA纯度最高,但浓度最低且提取时间最长;改良SDS法提取DNA浓度最高,所需时间较短,但纯度较低;改良CTAB法Ⅲ提取所需时间最短。[结论]建立了铁线莲最适DNA提取方法,为运用分子生物学手段对其研究提供支持。

关 键 词：Clematis fasciculiflora Franch Extraction of genomic DNA Improved CTAB method Improved SDS method 

分 类 号：S567.19[农业科学—中草药栽培]