Expression of Recombinant Gene P1-2A3C of FMDV Asia I in Different Bombyx mori Varieties  

Asia I口蹄疫病毒组合基因P1-2A3C在不同家蚕品种中的表达(英文)

作　　者：张韵[1] 张金卫 易咏竹[3] 李志勇[1] 李江涛[1] 鱼南洋[1] 丁农[1] 柳纪省[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室,甘肃兰州730046 [2]湖州市农业科学研究院,浙江湖州313000 [3]中国农科院生物技术所,北京100081

出　　处：《Agricultural Science & Technology》2011年第10期1528-1530,共3页农业科学与技术（英文版）

基　　金：Supported by863Project for Development and Production of New FMDV accine for Livestock(2011AA10A211);Fund for Agricultural Since and Technology Achievements Transformation(2009GB23260437)~~

摘　　要：[Objective] The aim was to investigate the expression level of recombinant gene P1-2A3C of FMDV Asia I in different Bombyx mori varieties,so as to screen out the B.mori varieties suitable for the foreign gene expression.[Method] The recombinant B.mori baculovirus rBmNPV（P1-2A3C） are injected into pupae of original species and hybrids of B.mori respectively.Then,the expression of antigen is detected by ELISA method and compared.[Result] The expression level of P1-2A3C significantly differed in different B.mori varieties,in which,the hybrids of Qiufeng×TQ78 and Qiufeng×Sijiaojian could be considered as the effective bioreactors for high-level expression of foreign genes.[Conclusion] This study provided the basis for breeding special B.mori varieties which can highly express target protein of Asia I FMDV.[目的]研究AsiaI口蹄疫病毒组合基因P1-2A3C在不同家蚕品种中的表达,以期筛选出较适合表达该组合基因的家蚕品种。[方法]将已经获得高效表达的重组家蚕杆状病毒rBmNPV(P1-2A3C)基因,分别注射原种及杂交家蚕蚕蛹。利用ELISA方法对其进行抗原表达量检测,并对表达结果进行差异比较。[结果]不同家蚕品种对rBmNPV(P1-2A3C)的表达存在着明显差异;秋丰×TQ78和秋丰×丝胶茧杂交组合可考虑作为高效表达的家蚕生物反应器专用品种。[结论]为AsiaIFMDV目的蛋白的高效表达专用品种的选育提供了依据。

关 键 词：Asia I FMDV P1-2A3C Bombyx mori varieties 

分 类 号：S852.65[农业科学—基础兽医学] S882[农业科学—兽医学]