Research on the Lectin in Muscle Tissue of Varicorhinus macrolepis  

泰山赤鳞鱼肌肉中凝集素的研究(英文)

作　　者：庞秋香[1] 赵先锋[1] 孙欢欢[1] 赵博生[1] 孙皓[1] 

机构地区：[1]山东理工大学生命科学院,山东淄博255049

出　　处：《Agricultural Science & Technology》2011年第10期1535-1538,共4页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(30700071;31172074);National Natural Science Foundation of Shandong Province(ZR2010CL002)~~

摘　　要：[Objective] The paper aimed at researching lectins in muscles of Varicorhinus macrolepis and providing scientific basis for researching the adaptation mechanism and immune response of V.macrolepis to environment,which were advantageous for the protection and reproduction of V.macrolepis.[Method] V.macrolepis was used as test materials for the hemagglutination test by dialdehyde fixation to prove the existence of lectins in muscle crude homogenate of Varicorhinus macrolepis and study the physical and chemical characters.[Result] Lectins in muscle crude homogenate of V.macrolepis had shown hemagglutination effects on erythrocytes of six types of animals and had the maximum hemagglutination activity against rabbit erythrocytes,which belonged to the S-type lectins with optimal pH ranged from 4 to 8 and optimal temperature at 60 ℃.Results from the saccharide inhibition test had indicated that the sucrose was the only kind of saccharide which had inhibited the hemagglutination,suggesting that sucrose had played an important role in the process of recognition and aggregation of lectins.[Conclusion] It had been speculated that the optimal pH ranges for thermal sensitivity and hemagglutination activity of lectins in different types of aquatic organisms were similar.[目的]研究泰山赤鳞鱼肌肉中的凝集素,旨在为赤鳞鱼对环境的适应机制和免疫反应提供科学依据,从而有利于对泰山赤鳞鱼的保护与繁殖。[方法]以泰山赤鳞鱼为试验材料,运用双醛固定的血细胞凝集试验证实在泰山赤鳞鱼肌肉提取液中的存在凝集素并对其部分理化性质进行研究。[结果]赤鳞鱼肌肉提取液凝集素对6种动物血细胞都有凝集作用,其中对兔血红细胞的凝血活性效价最高;该凝集素属于巯基依赖型凝集素,其最适pH为4～8,最适温度是60℃;糖抑制试验显示只有蔗糖对其凝集有抑制作用,说明蔗糖在该凝集素的识别和凝集过程起重要作用。[结论]推测在不同种类的水生生物中存在的凝集素对热敏感性及发生凝集活性的适宜pH范围是相似的。

关 键 词：V.macrolepis Muscle crude homogenate LECTIN Dialdehyde fixation Hemagglutination activity 

分 类 号：S917.4[农业科学—水产科学]