黑米花青素对大鼠视网膜光化学损伤感光细胞凋亡和Caspase-1表达的影响  

The effects of black rice anthocyanins on apoptosis and caspase-1 expression in photochemical damaged retinas in rats

作　　者：陈玮[1] 贾皓[1] 余小平[1] 伍秀华[1] 李帅[1] 刘洪[1] 廖纪如[1] 凌文华[2] 

机构地区：[1]成都医学院公共卫生系,四川成都610083 [2]中山大学公共卫生学院,广东广州510080

出　　处：《成都医学院学报》2011年第3期196-199,共4页Journal of Chengdu Medical College

基　　金：国家自然科学基金(No:30972462);教育部新世纪优秀人才支持计划(No:NECT-08-0901);教育部重点项目(No:208123)

摘　　要：目的探讨黑米花青素(black rice anthocyanins,BRACs)对视网膜光化学损伤(retinal photochemical damage,RPD)感光细胞凋亡及Caspase-1表达的影响。方法 60只Sprague-Dawley大鼠随机分为对照组(n=30)和BRACs组(n=30),均饲以基础饲料,12h明/12h暗循环光照。BRACs组按100mg/kg.bw剂量给予BRACs,每天灌胃1次;对照组给予等量生理盐水灌胃1次。15d后,两组动物同时给予(3 000±200)lux强度的白色荧光持续光照0～24h,原位末端标记(TUNEL)法检测视网膜细胞凋亡,逆转录聚合酶链反应(RT-PCR)法和免疫组织化学法检测视网膜内Caspase-1mRNA和蛋白表达水平。结果光照能诱导大鼠视网膜感光细胞凋亡(P<0.05),灌胃给予BRACs能降低光照诱导的感光细胞凋亡(P<0.05);光照后Caspase-1mRNA和蛋白表达增加,BRACs干预后Caspase-1表达显著降低(P<0.05);光照后外核层和内核层出现大量的Caspase-1阳性细胞,而BRACs组阳性细胞数量明显少于对照组。结论 BRACs能抑制RPD中感光细胞的凋亡,防护视网膜光化学损伤,该作用可能与其下调Caspase-1表达相关。Objective To investigate the effects of black rice anthocyanins（BRACs） on apoptosis and expression of caspase-1 mRNA and protein in photochemical damaged retinas.Methods Sixty Sprague-Dawley rats were randomly divided into control group（n=30） and BRACs group（n=30）,and fed with basic diet with a 12 hous light-dark cycle.The BRACs group was administered with BRACs by stomach intubation at a dosage of 100 mg/kg·bw for 15 days.Meanwhile,the control group was administered with equal volume physiological saline without BRACs.Then the two groups were exposed to（3000±200） lux fluorescent light for 0-24 hous.Thereafter,the apoptosis of photoreceptors was assessed by terminal-deoxynucleoitidyl transferase mediated nick end labeling（TUNEL）,and the expression of caspase-1 mRNA and protein in photochemical damaged retinas was determined by RT-PCR and immunohistochemistry,respectively.Results The apoptotic photoreceptors were increased markedly after light exposure（P0.05）,and BRACs suppressed photoreceptors degeneration（P0.05）.The expression level of caspase-1 mRNA and protein was increased in light damaged retinas,and BRACs down-regulated caspase-1 expression（P0.05）.After light exposure,there were considerable caspase-1 positive cells,but there was a decrease in BRACs group compared to the control（P0.05）.Conclusion BRACs can inhibit photoreceptor apoptosis,which maybe is related with the down-regulation of apoptotic proteins caspase-1.

关 键 词：黑米花青素 细胞凋亡 CASPASE-1 大鼠 视网膜光化学损伤 

分 类 号：R395.4[哲学宗教—心理学]