蓖麻毒素基本生物学特性分析  

Analysis on Basic Biological Characteristics of Ricin Toxin

作　　者：刘林娜[1,2] 董瓛 高宏伟[1,2] 李吉平[1,2] 孙玉成 刘文森[1,2] 万家余 钱军[1,2] 

机构地区：[1]军事医学科学院军事兽医研究所,吉林长春130022 [2]吉林省人兽共患病预防与控制重点实验室,吉林长春130022 [3]吉林农业大学动物科技学院,吉林长春130118

出　　处：《安徽农业科学》2011年第28期17129-17131,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(31101858)

摘　　要：[目的]对蓖麻毒素分子量、等电点和氨基酸序列等基本生物学特性进行分析。[方法]以内蒙古通辽地区所产蓖麻哲蓖2号为对象,利用毛细管电泳技术、分子生物学技术、毒性检测等免疫学技术对其毒素基本生物学特性进行检测分析。[结果]试验中所用蓖麻毒素的等电点为6.49,分子量为61.9kD,其氨基酸序列与网上公布序列相比,A链有22个位点发生变异,B链无变异;小鼠口服LD50为5.0mg/kg,腹腔注射LD50为4.6μg/kg,淋巴细胞IC50为3μg/ml。[结论]该研究为深入了解蓖麻毒素生物学性质提供依据,同时为不同产地蓖麻毒素毒性及其他性质的差异比较奠定基础。[Objective] The basic biological characteristics of ricin toxin,such as molecular weight（MW）,isoelectric point（pI）,amino acid sequence were analyzed.[Method] With Zhebi No.2 from Tongliao Region of Inner Mongolia as the object,capillary electrophoresis（CE） technology,molecular biological and immunological technology were adopted to analyze the basic biological characteristics of ricin toxin.[Result] The MW is 61.9 kD,pI is 6.49.For its amino acid sequence,A chain of ricin has 22 sites varied and B chain has no mutation compared with the sequence of publication in NCBI.For its toxicity,towards to BALB/c mice,LD50 of oral administration is 5.0 mg/kg,i.p is 4.6 μg/kg and IC50 of lymphocytes is 3 μg/ml.[Conclusion] The study will provide basis for deeply understanding the biological characteristics,and lay a foundation for comparison of ricin toxin from various places.

关 键 词：蓖麻毒素 分子量 等电点 氨基酸序列 毒性 

分 类 号：S132[农业科学—农业基础科学]