隐藏嗜酸菌Acidiphilium cryptum JF-5分泌蛋白质组的分析  

Analysis of Signal Peptide of the Secreted Protein in Genome of Acidiphilium Cryptum JF-5

作　　者：焦春香[1] 张奉波[2] 夏金兰[2] 张成桂[1,2] 

机构地区：[1]大理学院,云南大理671000 [2]中南大学资源加工与生物工程学院,湖南长沙410083

出　　处：《安徽农业科学》2011年第28期17168-17171,17175,共5页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(50974140)

摘　　要：[目的]对隐藏嗜酸菌(Acidiphilium cryptumJF-5)全基因组的3063个氨基酸序列进行预测,分析其分泌蛋白质组的构成。[方法]利用信号肽预测软件SignalPv3.0、跨膜螺旋结构预测软件TMHMMv2.0、RR-motif信号肽预测软件TatPv1.0、脂蛋白质信号肽预测软件LipoPv1.0、非经典分泌蛋白质预测软件SecretomeP和亚细胞定位软件Psortbv2.0.4对Acidiphilium cryptumJF-5全基因组的3063个氨基酸序列进行预测分析。[结果]在隐藏嗜酸菌Acidiphilium cryptumJF-5中有444个蛋白质为分泌蛋白质,其中分泌型信号肽148个,RR-motif亚组型信号肽27个,脂蛋白质信号肽22个,无Prepiplin-like信号肽,非经典分泌蛋白质247个;另外,对分泌型信号肽的长度分布、氨基酸使用频率和酶切位点的氨基酸使用频率作出了统计分析。[结论]研究结果为进一步研究嗜酸菌Acidiphilium cryptumJF-5的基因功能提供了丰富的信息基础。[Objective] The component of the secreted protein from Acidiphilium cryptum JF-5 genome was analyzed through the prediction of 3063 amino acid sequences in it.[Method] The internet-based software SignalP v3.0,TMHMM v2.0,TatP v1.0,LipoP v1.0,SecretomeP and Psortb v2.0.4 were combined to predict the signal peptides from the 3063 ORFs in Acidiphilium cryptum JF-5.[Results] The results showed that total 444 proteins tested in Acidiphilium cryptum JF-5 were the secreted protein,among which,148 proteins was Sec-type signal peptide;27 proteins,RR-motif signal peptides;22 proteins,lipoprotein signal peptides,247 proteins,non-classical protein and no Prepilin-like signal peptide was found.Additionally,the length of the Sec-type signal peptide,the frequency of amino acid working,the working frequency of particular amino acid in restriction sites were statistically analyzed.[Conclusion] The rich information for the study on the function of Acidiphilium cryptum JF-5 genome was provided.

关 键 词：隐藏嗜酸菌Acidiphilium cryptumJF-5 分泌蛋白质组 信号肽 

分 类 号：X703.1[环境科学与工程—环境工程]