澳大利亚小麦品种高分子量谷蛋白亚基组成分析  

Composition Analysis of HMW-GS in Australian Wheat Cultivars

作　　者：傅晓艺[1] 贾丹[1] 李孟军[1] 何明琦[1] 史占良[1] 蔡欣[1] 郭进考[1] 

机构地区：[1]石家庄市农林科学研究院,河北石家庄050041

出　　处：《安徽农业科学》2011年第28期17209-17210,17264,共3页Journal of Anhui Agricultural Sciences

基　　金：国家小麦现代农业产业技术体系项目(CARS-03);国家973项目(2009CB118300)

摘　　要：[目的]为我国小麦品质育种提供有益的信息。[方法]利用SDS-PAGE技术对澳大利亚64份主要推广小麦品种的高分子量谷蛋白亚基(HMW-GS)组成进行研究,明确其HMW-GS构成,从中筛选出具有优质亚基的材料。[结果]在参试材料中共检测到9种不同的亚基类型。在Glu-A1位点有Null、1等位变异类型,以1为主要类型,频率为59.37%;Glu-B1有7、7+8、7+9、14+15、17+185个等位变异类型,以7+8为主要类型,频率为56.20%;Glu-D1有2+12、5+10等位变异类型,以2+12为主要类型,频率为70.30%。同时发现共有12种亚基组合,以"1,7+8,2+12"为主要类型。所有品种品质得分范围为4～10,平均得分为7.4。[结论]64个供试品种品质普遍较好。[Objective] The aim was to provide information for the breeding of wheat in China.[Method] 64 wheat cultivars from Australia were analyzed by SDS-PAGE to determine their high molecular weight（HMW） glutenin subunit combinations.Then,the cultivars had good HMW-GS were screened.[Result] A total of nine alleles were identified at Glu-1.Glu-A1 encoded two subunits,1 and null subunit,and 1 was the major type,the frequency was 59.37%;Glu-B1 encoded five subunits,7,7＋8,7＋9,14＋15,17＋ 18,and 7＋8 was the major type,the frequency was 56.20%;Glu-D1 encoded two subunits,2＋12,5＋10,and 2＋12 was the major type,the frequency was 70.30%.Furthermore,12 subunit combinations were detected,and the composition of ＂1,2＋12,7＋8＂ was the major type.The quality scores of these cultivars ranged from 4 to 10,with an average of 7.4.[Conclusion] The quality of these varieties was good.

关 键 词：澳大利亚:小麦 高分子量谷蛋白亚基 

分 类 号：S512.1[农业科学—作物学]