戊二醛对气单胞菌的抑菌杀菌效果研究  

Effect of the Glutaraldehyde on the Bacteriostatic and Bactericide of Aeromonas

作　　者：谭凤霞[1] 陈和东[1] 叶聪[1] 

机构地区：[1]长江大学动物科学学院,湖北荆州434025

出　　处：《安徽农业科学》2011年第28期17340-17341,17349,共3页Journal of Anhui Agricultural Sciences

基　　金：长江大学博士启动基金(801200010110)

摘　　要：[目的]研究戊二醛对气单胞菌的杀菌效果,为戊二醛在水产养殖上的合理使用提供依据。[方法]以气单胞菌属标准株ATCC7966和分离自养殖环境及患病鱼的气单胞菌为试验菌株,通过肉汤二倍稀释法研究。[结果]戊二醛在低浓度时能抑制气单胞菌的生长增殖,高浓度杀灭细菌,其对7株气单胞菌的最小抑菌浓度(MIC)均为7.8 mg/L,其最小杀菌浓度为62.5 mg/L(ATCC7966、Aero-monas sp.T3、Aeromonas sp.T5、Aeromonas sp.T6)和125mg/L(Aeromonas sp.T1、Aeromonas sp.T2、Aeromonas sp.T4)。[结论]为戊二醛的合理使用提供一定的依据。[Objective] The basis of the reasonable utilization of glutaraldehyde in aquaculture production was provided through the research on its effect on the bactericide of Aeromonas.[Method] The standard strain of Aeromonas--ATCC7966 and the Aeromonas strain isolated from fish-raising environment or from diseased fish was taken as the tested strain and the efficacy of the glutaraldehyde on the bacteriostatic and bactericide of Aeromonas was determined with the method of double broth dilution.[Results] The results showed that the glutaraldehyde could inhibit the growth of bacterium at the low concentration and kill the bacterium at the high concentration.The minimal inhibitory concentration（MIC） of glutaraldehyde to 7 Aeromonas strains was 7.8mg/l and the minimal bactericidal concentration（MBC） of glutaraldehyde to 4 Aeromonas strains including ATCC7966,Aeromonas sp.T3,Aeromonas sp.T5 and Aeromonas sp.T6 was 62.5 mg/l and 3 Aeromonas strains including Aeromonas sp.T1,Aeromonas sp.T2 and Aeromonas sp.T4 was 125mg/l.[Conclusion] The experimental results could provide the scientific basis of the reasonable application of glutaraldehyde.

关 键 词：戊二醛 气单胞菌 最小抑菌浓度(MIC) 最小杀菌浓度(MBC) 

分 类 号：S96[农业科学—水产养殖]