葡萄应答霜霉病过程中多磷酸肌醇激酶基因与过氧化氢的作用机制  

The mechanism of VvIPK2 and H_2O_2 in resistance of grapevine against downy mildew

作　　者：高超[1] 侯丽霞[1] 郭秀萍[1] 赵方贵[1] 刘新[1] 

机构地区：[1]青岛农业大学,山东省高等学校植物技术重点实验室,青岛266109

出　　处：《植物保护学报》2011年第5期443-448,共6页Journal of Plant Protection

基　　金：农业部“948”项目(2006-G26);青岛市自然科学基金(10-3-4-5-5-jch)

摘　　要：以对霜霉病不同抗性的葡萄品种左优红和霞多丽为材料,利用分子生物学和植物生理学试验手段,结合药理学试验,探讨葡萄在应答霜霉病过程中葡萄多磷酸肌醇激酶基因(VvIPK2)和H2O2的作用机制。接种霜霉病菌后15 h葡萄叶片VvIPK2表达量是正常水平的12倍,接种后3 hH2O2含量达最大值,同时苯丙氨酸解氨酶和几丁质酶活性升高;多磷酸肌醇激酶(IPK2)抑制剂、外源H2O2及H2O2清除剂均能改变霜霉病菌所引起的抗性葡萄品种左优红叶片PAL和几丁质酶活性的变化,同时可以影响不同抗性品种叶片的感病情况;IPK2抑制剂对葡萄霜霉病菌引起的H2O2水平变化没有影响;清除H2O2可减弱葡萄霜霉病菌对VvIPK2表达量的诱导效应。研究表明H2O2位于IPK2的上游,通过调控PAL和几丁质酶活性参与葡萄应答霜霉病过程。Two grapevine cultivars with different resistance to lasmopara viticola-Zuoyouhong,Chardonnay were taken as materials in this study.Through molecular biology,plant physiology and pharmacology experiment approaches,the roles of inositol polyphosphate kinase（VvIPK2） and hydrogen peroxide（H_2O_2） after inoculating P.viticola were studied.The results showed that the expression pattern of VvIPK2 was enhanced to 12 times at 15 hours,the level of H_2O_2 reaches to maximum value at 3 hours and the activity of PAL and chitinase increased in the leaves after being inoculated;inositol polyphosphate kinase（IPK2） inhibitor,H_2O_2 scavenger or exogenous H_2O_2 were able to change the activity of PAL and chitinase and the infective rate of downy mildew in leaves of resistant cultivar-Zuoyouhong infected by P.viticola.At the same time IPK2 inhibitor failed to induce the change of H_2O_2 induced by P.viticola.However,the expression pattern of VvIPK2 was weakened when H_2O_2 was scavenged.It was concluded that H_2O_2 regulated the expression pattern of VvIPK2,then relative enzymes activity so as to enhance the resistance of grapevine against downy mildew.

关 键 词：葡萄 霜霉病 多磷酸肌醇激酶基因 过氧化氢 

分 类 号：S436.631.1[农业科学—农业昆虫与害虫防治]