稻瘟病菌对稻瘟灵、异稻瘟净和三环唑的敏感性  

Sensitivities of Magnaporthe grisea to isprothiolane,iprobenfos and tricyclazole

作　　者：杜宜新[1] 李科[1] 阮宏椿[1] 杨秀娟[1] 甘林[1] 陈福如[1] 

机构地区：[1]福建省农业科学院植物保护研究所,福州350013

出　　处：《植物保护学报》2011年第5期455-460,共6页Journal of Plant Protection

基　　金：福建省科技厅公益性项目(2009R10028-4);福建省科技创新平台建设项目(2009N2005)

摘　　要：采用菌丝生长速率法分别测定了57株稻瘟病菌对稻瘟灵和79株稻瘟病菌对异稻瘟净的敏感性,分别建立了敏感基线,并检测了141株大田稻瘟病菌对稻瘟灵和异稻瘟净的抗药性水平;采用活体植株法测定了223株稻瘟病菌对三环唑的敏感性。57株稻瘟病菌对稻瘟灵的平均EC50值为1.4312±0.0867μg/mL,其敏感性频率分布呈连续性单峰曲线,1.4312μg/mL可作为稻瘟病菌对稻瘟灵的敏感基线,在141株大田稻瘟病菌中敏感、低抗和中抗菌株出现的频率分别为21.99%、73.76%和4.26%;79株稻瘟病菌对异稻瘟净的平均EC50值为6.6393±0.2662μg/mL,其敏感性频率分布也呈连续性单峰曲线,6.6393μg/mL可作为稻瘟病菌对异稻瘟净的敏感基线,141株大田稻瘟病菌中敏感、低抗和中抗菌株出现的频率分别为90.07%、7.80%和2.13%。稻瘟病菌对三环唑的EC50值最大为47.6144μg/mL,最小为1.0850μg/mL,供试菌株的EC50值呈近似正态分布,未出现敏感性下降的抗药性亚群体。The sensitivity of the 57 Magnaporthe grisea strains to isoprothiolane and the sensitivity of the 79 M.grisea strains to iprobenfos were established with mycelium growth rate method,and the sensitivities of 141 isolates from fields were determined to monitor the resistance to isoprothiolane and iprobenfos.The sensitivity of the 223 M.grisea strains to tricyclazole was detected in vivo.The results showed that the EC_50 of isoprothiolane and iprobenfos to M.grisea were 1.4312±0.0867μg/mL and 6.6393±0.2662μg/mL,which showed a unimodal distribution.Thus,1.4312μg/mL and 6.6393μg/mL could be used as baseline-sensitivity of M.grisea to isoprothiolane and iprobenfos for field resistance monitoring,respectively.And the frequencies of sensitive,low-resistant and intermediate-resistant isolates from fields to isoprothiolane were 21.99%,73.76% and 4.26%,to iprobenfos were 90.07%,7.80% and 2.13%,respectively.All the 223 isolates were sensitive to tricyclazole with EC_50 ranging from 1.0850 to 47.6144μg/mL,which showed a skewed unimodal distribution,and no resistant subpopulation was detected among these strains.

关 键 词：稻瘟病菌 稻瘟灵 异稻瘟净 三环唑 抗药性 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]