HPLC法同时测定千斤拔属植物7种黄酮的含量  

Simultaneous Determination of Seven Flavonoids in the Radix of Flemingia by HPLC

作　　者：李莉[1,2] 刘志华[3] 秦民坚[1,2] 

机构地区：[1]中国药科大学中药资源学研究室,江苏南京210009 [2]中国药科大学教育部现代中药研究重点实验室,江苏南京210009 [3]中国医学科学北京协和研究院药用植物研究所,北京100193

出　　处：《中国野生植物资源》2011年第5期54-57,65,共5页Chinese Wild Plant Resources

摘　　要：目的:测定千斤拔属7种植物中的7种黄酮类的含量,为千斤拔类药材质量控制提供依据。方法:色谱条件为Hedera C18色谱柱(250 mm×4.6 mm,5.0μm):流速1.2 mL/min,流动相为0.5%HAc水和乙腈梯度洗脱,柱温35℃,检测波长263 nm。结果:七种黄酮类成分在90 min内达到现行分离,线性关系良好(,R2>0.9995),日内、日间精密度、稳定性和重复性的RSD≤3.0%。该法成功的应用于千斤拔药材7种黄酮的评均回收率分别为96.94%～105.29%(RSD≤2.98%)结论:本方法准确、快速、重现性好,不同品种的千斤拔药材质量存在差异,可以为研究千斤拔药材的质量标准提供依据。Objective： To develop a High-Performance Liquid Chromatography（HPLC） method for the simultaneous determination of the radix of Flemingia.Method： The separation was carried out on a Hedera ODS（4.6 mm×250 mm,5 μm） column eluted with in mobile phases of water containing 0.5% HAc and Acetonitrile gradient mode at the flow rate of 1.2 mL/min.The detective wavelength was 263nm and column temperature was 35 ℃.Results： The good separation of seven flavonoids was achieved within 90 min and showed good linear relationship（R20.9995）,with the RSD of intra-day and inter-day presision,repeatability and stability3.0%.The average recoveries of the seven flavonids were within 96.94%-105.29%（RSD≤2.98%）.Conculsion： The developed method is simple,accurate and repeatable,and can be readily used as a powerful tool for the quality control of Flemingia.

关 键 词：千斤拔 HPLC 黄酮 含量测定 

分 类 号：R284.1[医药卫生—中药学]