1,4-二氯苯对小白菜种子萌发和幼苗的毒性  

Toxicity of 1,4-Dichlorobenzene on Seed Germination and Seedling Growth of Three Pai-Tsai(Brassica chinensis L.)

作　　者：石瑛[1] 杜青平[2] 李砧[1] 

机构地区：[1]太原师范学院生物系,山西太原030031 [2]广东工业大学环境科学与工程学院,广东广州510006

出　　处：《环境科学研究》2011年第10期1178-1184,共7页Research of Environmental Sciences

基　　金：国家自然科学基金项目(40971251);山西省高校高新技术产业化项目(2010019);山西省基础研究项目(青年基金)(2010021027-1)

摘　　要：采用室内模拟染毒法,研究1,4-DCB(1,4-二氯苯)对3种小白菜种子(矮青6号、上海青和秦白2号)萌发和蔬菜幼苗的毒性作用.结果表明:1,4-DCB作用后,3种小白菜种子的发芽势、种子发芽率、发芽指数和活力指数均降低,并呈现一定的剂量-效应关系;种子受1,4-DCB胁迫后,小白菜幼苗的根和苗生长都受到了抑制,根长和苗高降低,根苗比显著下降;随着ρ(1,4-DCB)的升高,受胁迫后的幼苗叶片的w(游离脯氨酸)呈现先升高后降低的趋势,而MDA(丙二醛)含量逐渐升高,与ρ(1,4-DCB)呈正相关;幼苗叶片SOD(超氧化物歧化酶)活性在ρ(1,4-DCB)为5 mg/L时升高,在ρ(1,4-DCB)为10和50 mg/L时明显降低;与对照组相比,各处理组幼苗叶片POD(过氧化物酶)活性均升高,但是ρ(1,4-DCB)为50 mg/L时酶活性比ρ(1,4-DCB)为10 mg/L时下降.表明水体中1,4-DCB污染对蔬菜种子萌发和幼苗生长产生毒性,其毒性机制与膜脂质过氧化有关.The effects of 1,4-dichlorobenzene（1,4-DCB） on seed germination and seedling toxicity of three pai-tsai（Brassica chinensis L.）（Aiqing #6,Shanghaiqing,Qinbai #2） were studied through simulation experiments in a laboratory.The results showed that the germination energies and germination rates of the three pai-tsai seeds in the groups treated with 1,4-DCB were decreased in a concentration-response manner,as were germination and vigor indices.The lengths of the roots and the heights of the seedlings were decreased when the pai-tsai seeds were treated by 1,4-DCB.Furthermore,the inhibition rates for the seedling roots were higher than those for the shoots.With the increasing of 1,4-DCB treatment concentrations,the proline contents of the seedling leaves increased at lower concentrations（5,10 mg/L）,then decreased at higher concentrations（50 mg/L）.The malondialdehyde（MDA） contents in the leaves gradually accumulated in a dose-response manner.The superoxide dismutase（SOD） activities in the seedling leaves increased at 5 mgL,and decreased at 10 mgL and 50 mg/L treatment of 1,4-DCB.Compared with the control group,the peroxidase（POD） activities in the seedling leaves of the treated groups were increased.However,the POD activities of the 50 mg/L groups were lower than those of the 10 mgL groups.The results indicated that the pollution of 1,4-DCB in the water would be harmful to vegetable seed germination and seedling growth.The primary damage mechanism of 1,4-DCB on vegetable seeds and seedlings may be related to the lipid peroxidation of the biomembrane of the pai-tsai seedling cells.

关 键 词：1 4-二氯苯 蔬菜种子 萌发 毒性 

分 类 号：X59[环境科学与工程—环境工程]