两株海洋细菌新种的分离及BOX-PCR鉴定  

Isolation and BOX-PCR identification of two new species of marine bacteria

作　　者：朱凤玲[1,2] 曲凌云[1,2] 洪旭光[1,2] 孙修勤[1,2] 

机构地区：[1]国家海洋局第一海洋研究所,山东青岛266061 [2]国家海洋局海洋生态环境科学与工程重点实验室,山东青岛266061

出　　处：《海洋环境科学》2011年第5期622-625,共4页Marine Environmental Science

基　　金：国家科技基础平台工作项目(2004DKA30640);国家自然科学青年科学基金(41006103);国家海洋局第一海洋研究所基本科研业务费专项资金项目(GY02-2011G24)

摘　　要：对即墨海参养殖池及即墨盐场的底泥样本进行微生物的可培养研究,获得的两株细菌DQHS13与YCSA40,其与亲缘关系最近的模式菌株Marinobacter zhejiangensis及M.segnicrescens的16S rDNA序列相关性分别为98.789%和96.963%,初步判定为海杆菌属(Marinobacter)的新种。采用BOX-PCR技术分别对这些菌株与其亲缘关系最近的模式菌株进行比较,证实了它们与模式菌株之间DNA指纹图谱存在差异,为下一步的研究提供了一定帮助。Two marine bacteria,DQHS13 and YCSA40,were isolated from sediment of sea cucumber farm and saltwoks in Jimo.Phylogenetic analysis based on 16S rDNA gene sequences showed that strain DQHS13 and YCSA40 were probably new species of the genus Marinobacter,and it also showed that the previously known member of the genus Marinobacter zhejiangensis and M.segnicrescens sp.nov.were the nearest neighbors to strain DQHS13 and YCSA40.The levels of sequence similarity between them are 98.789% and 96.963%.BOX-PCR fingerprint showed the differences between the two strains and their type strains.

关 键 词：BOX-PCR 海杆菌新种 鉴定 

分 类 号：Q331[生物学—遗传学]